Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238
Polyhydroxybutyrate (PHB) was produced by Cupriavidus necator CCGUG 52238 using organic acids from fermented kitchen waste. HPLC and nuclear magnetic resonance (NMR) analyses revealed that the acid comprised mainly of lactic and acetic acids. In shake flask culture, the lactic acid concentration abo...
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22363/ http://psasir.upm.edu.my/id/eprint/22363/1/Utilization%20of%20kitchen%20waste%20for%20the%20production%20of%20green.pdf |
| _version_ | 1848844465178935296 |
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| author | Omar, Farah Nadia Abdul Rahman, Nor'aini Hafid, Halimatun Saadiah Mumtaz, Tabassum Phang, Lai Yee Hassan, Mohd Ali |
| author_facet | Omar, Farah Nadia Abdul Rahman, Nor'aini Hafid, Halimatun Saadiah Mumtaz, Tabassum Phang, Lai Yee Hassan, Mohd Ali |
| author_sort | Omar, Farah Nadia |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Polyhydroxybutyrate (PHB) was produced by Cupriavidus necator CCGUG 52238 using organic acids from fermented kitchen waste. HPLC and nuclear magnetic resonance (NMR) analyses revealed that the acid comprised mainly of lactic and acetic acids. In shake flask culture, the lactic acid concentration above 10 g/L inhibited both cell growth and polyhydroxybutyrate (PHB) production. The PHB production by the strain was achieved at the highest PHB content of 52.79% in batch fermentation using the kitchen-waste derived organic acids. The PHB yield and productivity were 0.38 g/g and 0.065 g/L/h, respectively. In fed-batch culture, about 4-fold increase in PHB productivity (0.242 g/L/h) was achieved by applying intermittent feeding strategy. |
| first_indexed | 2025-11-15T08:31:21Z |
| format | Article |
| id | upm-22363 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:21Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223632017-11-17T08:08:45Z http://psasir.upm.edu.my/id/eprint/22363/ Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238 Omar, Farah Nadia Abdul Rahman, Nor'aini Hafid, Halimatun Saadiah Mumtaz, Tabassum Phang, Lai Yee Hassan, Mohd Ali Polyhydroxybutyrate (PHB) was produced by Cupriavidus necator CCGUG 52238 using organic acids from fermented kitchen waste. HPLC and nuclear magnetic resonance (NMR) analyses revealed that the acid comprised mainly of lactic and acetic acids. In shake flask culture, the lactic acid concentration above 10 g/L inhibited both cell growth and polyhydroxybutyrate (PHB) production. The PHB production by the strain was achieved at the highest PHB content of 52.79% in batch fermentation using the kitchen-waste derived organic acids. The PHB yield and productivity were 0.38 g/g and 0.065 g/L/h, respectively. In fed-batch culture, about 4-fold increase in PHB productivity (0.242 g/L/h) was achieved by applying intermittent feeding strategy. Academic Journals 2011-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22363/1/Utilization%20of%20kitchen%20waste%20for%20the%20production%20of%20green.pdf Omar, Farah Nadia and Abdul Rahman, Nor'aini and Hafid, Halimatun Saadiah and Mumtaz, Tabassum and Phang, Lai Yee and Hassan, Mohd Ali (2011) Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238. African Journal of Microbiology Research, 5 (19). art. no. E4B84EC13036. pp. 2873-2879. ISSN 1996-0808 http://www.academicjournals.org/journal/AJMR/article-abstract/E4B84EC13036 |
| spellingShingle | Omar, Farah Nadia Abdul Rahman, Nor'aini Hafid, Halimatun Saadiah Mumtaz, Tabassum Phang, Lai Yee Hassan, Mohd Ali Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238 |
| title | Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238 |
| title_full | Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238 |
| title_fullStr | Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238 |
| title_full_unstemmed | Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238 |
| title_short | Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238 |
| title_sort | utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (phb) by cupriavidus necator ccgug 52238 |
| url | http://psasir.upm.edu.my/id/eprint/22363/ http://psasir.upm.edu.my/id/eprint/22363/ http://psasir.upm.edu.my/id/eprint/22363/1/Utilization%20of%20kitchen%20waste%20for%20the%20production%20of%20green.pdf |