Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase
Enzyme immobilisation technology is an effective means to improve sugar ester production through the employment of biocatalysts. In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2012
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/22360/ http://psasir.upm.edu.my/id/eprint/22360/1/Biocatalytic%20production%20of%20lactose%20ester%20catalysed%20by%20mica.pdf |
| _version_ | 1848844464383066112 |
|---|---|
| author | Zaidan, Uswatun Hasanah Abdul Rahman, Mohd Basyaruddin Othman, Siti Salhah Basri, Mahiran Abd. Malek, Emilia Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar |
| author_facet | Zaidan, Uswatun Hasanah Abdul Rahman, Mohd Basyaruddin Othman, Siti Salhah Basri, Mahiran Abd. Malek, Emilia Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar |
| author_sort | Zaidan, Uswatun Hasanah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enzyme immobilisation technology is an effective means to improve sugar ester production through the employment of biocatalysts. In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). Free and immobilised lipases were tested for their esterification activities. Specific activities for Amino-CRL and NER-CRL increased by 2.4 and 2.6-fold, respectively, upon immobilisation. Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. The optimised conditions for sugar fatty acid ester syntheses are 48 h at 2:1 of molar ratio of lactose sugar to capric acid at 55 °C. Furthermore, a high operational stability with half-lives of over 13 and 10 runs was achieved for NER-CRL and Amino-CRL, respectively, indicating the efficiency of the immobilisation process. |
| first_indexed | 2025-11-15T08:31:20Z |
| format | Article |
| id | upm-22360 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:20Z |
| publishDate | 2012 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223602016-09-26T07:01:53Z http://psasir.upm.edu.my/id/eprint/22360/ Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase Zaidan, Uswatun Hasanah Abdul Rahman, Mohd Basyaruddin Othman, Siti Salhah Basri, Mahiran Abd. Malek, Emilia Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Enzyme immobilisation technology is an effective means to improve sugar ester production through the employment of biocatalysts. In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). Free and immobilised lipases were tested for their esterification activities. Specific activities for Amino-CRL and NER-CRL increased by 2.4 and 2.6-fold, respectively, upon immobilisation. Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. The optimised conditions for sugar fatty acid ester syntheses are 48 h at 2:1 of molar ratio of lactose sugar to capric acid at 55 °C. Furthermore, a high operational stability with half-lives of over 13 and 10 runs was achieved for NER-CRL and Amino-CRL, respectively, indicating the efficiency of the immobilisation process. Elsevier 2012-03-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22360/1/Biocatalytic%20production%20of%20lactose%20ester%20catalysed%20by%20mica.pdf Zaidan, Uswatun Hasanah and Abdul Rahman, Mohd Basyaruddin and Othman, Siti Salhah and Basri, Mahiran and Abd. Malek, Emilia and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2012) Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase. Food Chemistry, 131 (1). pp. 199-205. ISSN 0308-8146; ESSN: 1873-7072 10.1016/j.foodchem.2011.08.060 |
| spellingShingle | Zaidan, Uswatun Hasanah Abdul Rahman, Mohd Basyaruddin Othman, Siti Salhah Basri, Mahiran Abd. Malek, Emilia Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase |
| title | Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase |
| title_full | Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase |
| title_fullStr | Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase |
| title_full_unstemmed | Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase |
| title_short | Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase |
| title_sort | biocatalytic production of lactose ester catalysed by mica-based immobilised lipase |
| url | http://psasir.upm.edu.my/id/eprint/22360/ http://psasir.upm.edu.my/id/eprint/22360/ http://psasir.upm.edu.my/id/eprint/22360/1/Biocatalytic%20production%20of%20lactose%20ester%20catalysed%20by%20mica.pdf |