Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) granule formation in vivo in wild type Comamonas sp. EB172 grown in mixed organic acids under nitrogen limitation were visualized under transmission electron microscope (TEM). The early stages of PHBV production revealed dark-stained mediation elements ne...

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Main Authors: Mumtaz, Tabassum, Abd. Aziz, Suraini, Abdul Rahman, Nor'aini, Phang, Lai Yee, Wasoh @ Mohamad Isa, Helmi, Shirai, Yoshihito, Hassan, Mohd Ali
Format: Article
Language:English
Published: Taylor & Francis 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22355/
http://psasir.upm.edu.my/id/eprint/22355/1/Viscous%20flow%20due%20to%20a%20permeable%20stretching.pdf
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author Mumtaz, Tabassum
Abd. Aziz, Suraini
Abdul Rahman, Nor'aini
Phang, Lai Yee
Wasoh @ Mohamad Isa, Helmi
Shirai, Yoshihito
Hassan, Mohd Ali
author_facet Mumtaz, Tabassum
Abd. Aziz, Suraini
Abdul Rahman, Nor'aini
Phang, Lai Yee
Wasoh @ Mohamad Isa, Helmi
Shirai, Yoshihito
Hassan, Mohd Ali
author_sort Mumtaz, Tabassum
building UPM Institutional Repository
collection Online Access
description Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) granule formation in vivo in wild type Comamonas sp. EB172 grown in mixed organic acids under nitrogen limitation were visualized under transmission electron microscope (TEM). The early stages of PHBV production revealed dark-stained mediation elements near the center of the cell supporting the third model of granule formation. The native granules revealed characteristic core-shell structure in which white PHB cores were surrounded by darker PHBV shells. In vitro, the copolymer showed bimodal molecular weight distribution and exhibited two melting peaks in the differential scanning calorimeter (DSC) thermogram, supporting the formation of block copolymer.
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institution Universiti Putra Malaysia
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publishDate 2011
publisher Taylor & Francis
recordtype eprints
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spelling upm-223552016-09-02T08:10:54Z http://psasir.upm.edu.my/id/eprint/22355/ Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope Mumtaz, Tabassum Abd. Aziz, Suraini Abdul Rahman, Nor'aini Phang, Lai Yee Wasoh @ Mohamad Isa, Helmi Shirai, Yoshihito Hassan, Mohd Ali Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) granule formation in vivo in wild type Comamonas sp. EB172 grown in mixed organic acids under nitrogen limitation were visualized under transmission electron microscope (TEM). The early stages of PHBV production revealed dark-stained mediation elements near the center of the cell supporting the third model of granule formation. The native granules revealed characteristic core-shell structure in which white PHB cores were surrounded by darker PHBV shells. In vitro, the copolymer showed bimodal molecular weight distribution and exhibited two melting peaks in the differential scanning calorimeter (DSC) thermogram, supporting the formation of block copolymer. Taylor & Francis 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22355/1/Viscous%20flow%20due%20to%20a%20permeable%20stretching.pdf Mumtaz, Tabassum and Abd. Aziz, Suraini and Abdul Rahman, Nor'aini and Phang, Lai Yee and Wasoh @ Mohamad Isa, Helmi and Shirai, Yoshihito and Hassan, Mohd Ali (2011) Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope. International Journal of Polymer Analysis and Characterization, 16 (4). pp. 228-238. ISSN 1023-666X; ESSN: 1563-5341 10.1080/1023666X.2011.569990
spellingShingle Mumtaz, Tabassum
Abd. Aziz, Suraini
Abdul Rahman, Nor'aini
Phang, Lai Yee
Wasoh @ Mohamad Isa, Helmi
Shirai, Yoshihito
Hassan, Mohd Ali
Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope
title Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope
title_full Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope
title_fullStr Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope
title_full_unstemmed Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope
title_short Visualization of core/shell PHBV granules of wild type Comamonas sp. EB 172 in vivo under transmission electron microscope
title_sort visualization of core/shell phbv granules of wild type comamonas sp. eb 172 in vivo under transmission electron microscope
url http://psasir.upm.edu.my/id/eprint/22355/
http://psasir.upm.edu.my/id/eprint/22355/
http://psasir.upm.edu.my/id/eprint/22355/1/Viscous%20flow%20due%20to%20a%20permeable%20stretching.pdf