Basic characteristics of cellulase immobilized on lignophenol
Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-bas...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nihon Kobunshi Gakkai,Society of Polymer Science, Japan
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22349/ http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf |
| _version_ | 1848844461759528960 |
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| author | Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu |
| author_facet | Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu |
| author_sort | Nonaka, Hiroshi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable. |
| first_indexed | 2025-11-15T08:31:18Z |
| format | Article |
| id | upm-22349 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:18Z |
| publishDate | 2011 |
| publisher | Nihon Kobunshi Gakkai,Society of Polymer Science, Japan |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223492015-12-10T03:36:37Z http://psasir.upm.edu.my/id/eprint/22349/ Basic characteristics of cellulase immobilized on lignophenol Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. Since the cost of cellulase has a big impact on the price of the products, it is important to develop cheaper alternatives. Here we report on the production of a novel immobilized cellulase. A functional lignin-based phenolic polymer “lignophenol” (p-cresol type : ligno-p-cresol) was synthesized from Hinoki wood meal and p-cresol through the phase separation system developed for selective separation of lignocellulosic components. Commercial cellulase derived from Trichoderma reeseiwas easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Immobilization reached more than 100 mg/g-lignophenol with an enzymatic activity of about 60% compared to free cellulase. With lower immobilization loads, cellulase exhibited lower activity. Cellulase did not significantly detach from lignophenol by mixing with acetate buffer, indicating that the cellulase immobilized by a simple physical adsorption is stable. Nihon Kobunshi Gakkai,Society of Polymer Science, Japan 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf Nonaka, Hiroshi and Ariffin, Hidayah and Funaoka, Masamitsu (2011) Basic characteristics of cellulase immobilized on lignophenol. Kobunshi Ronbunshu, 68 (5). pp. 315-319. ISSN 0386-2186 10.1295/koron.68.315 |
| spellingShingle | Nonaka, Hiroshi Ariffin, Hidayah Funaoka, Masamitsu Basic characteristics of cellulase immobilized on lignophenol |
| title | Basic characteristics of cellulase immobilized on lignophenol |
| title_full | Basic characteristics of cellulase immobilized on lignophenol |
| title_fullStr | Basic characteristics of cellulase immobilized on lignophenol |
| title_full_unstemmed | Basic characteristics of cellulase immobilized on lignophenol |
| title_short | Basic characteristics of cellulase immobilized on lignophenol |
| title_sort | basic characteristics of cellulase immobilized on lignophenol |
| url | http://psasir.upm.edu.my/id/eprint/22349/ http://psasir.upm.edu.my/id/eprint/22349/ http://psasir.upm.edu.my/id/eprint/22349/1/Basic%20characteristics%20of%20cellulase%20immobilized%20on%20lignophenol.pdf |