Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture
The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-α2b (PrIFN-α2b) was studied in shake flask culture and stirred tank bioreactor. Different types of anion-exc...
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| Format: | Article |
| Language: | English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22327/ http://psasir.upm.edu.my/id/eprint/22327/1/Enhanced%20production%20of%20periplasmic%20interferon%20alpha.pdf |
| _version_ | 1848844455645282304 |
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| author | Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya |
| author_facet | Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya |
| author_sort | Tan, Joo Shun |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-α2b (PrIFN-α2b) was studied in shake flask culture and stirred tank bioreactor. Different types of anion-exchange resin were evaluated and the concentration of anion-exchange resin was optimized using response surface methodology. The addition of anion-exchange resins reduced acetate accumulation in the culture, which in turn, improved growth of E. coli and enhanced PrIFN-α2b expression. The presence of anion-exchange resins did not influence the physiology of the cells. The weak base anion-exchange resins, which have higher affinity towards acetate, yielded higher PrIFN-α2b expression as compared to strong anion-exchange resins. High concentrations of anion-exchange resin showed inhibitory effect towards growth of E. coli as well as the expression of PrIFN-α2b. The maximum yield of PrIFN-α2b in shake flask culture (501.8 μg/L) and stirred tank bioreactor (578.8 μg/L) was obtained at ion exchange resin (WA 30) concentration of 12.2 g/L. The production of PrIFN-α2b in stirred tank bioreactor with the addition of ion exchange resin was about 1.8-fold higher than that obtained in fermentation without ion exchange resin (318.4 μg/L). |
| first_indexed | 2025-11-15T08:31:12Z |
| format | Article |
| id | upm-22327 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:12Z |
| publishDate | 2011 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223272016-03-15T00:44:26Z http://psasir.upm.edu.my/id/eprint/22327/ Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-α2b (PrIFN-α2b) was studied in shake flask culture and stirred tank bioreactor. Different types of anion-exchange resin were evaluated and the concentration of anion-exchange resin was optimized using response surface methodology. The addition of anion-exchange resins reduced acetate accumulation in the culture, which in turn, improved growth of E. coli and enhanced PrIFN-α2b expression. The presence of anion-exchange resins did not influence the physiology of the cells. The weak base anion-exchange resins, which have higher affinity towards acetate, yielded higher PrIFN-α2b expression as compared to strong anion-exchange resins. High concentrations of anion-exchange resin showed inhibitory effect towards growth of E. coli as well as the expression of PrIFN-α2b. The maximum yield of PrIFN-α2b in shake flask culture (501.8 μg/L) and stirred tank bioreactor (578.8 μg/L) was obtained at ion exchange resin (WA 30) concentration of 12.2 g/L. The production of PrIFN-α2b in stirred tank bioreactor with the addition of ion exchange resin was about 1.8-fold higher than that obtained in fermentation without ion exchange resin (318.4 μg/L). Elsevier 2011-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22327/1/Enhanced%20production%20of%20periplasmic%20interferon%20alpha.pdf Tan, Joo Shun and Ramanan, Ramakrishnan Nagasundara and Ling, Tau Chuan and Mustafa, Shuhaimi and Ariff, Arbakariya (2011) Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture. Biochemical Engineering Journal, 58-59. pp. 124-132. ISSN 1369-703X; ESSN: 1873-295X 10.1016/j.bej.2011.08.018 |
| spellingShingle | Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_full | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_fullStr | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_full_unstemmed | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_short | Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| title_sort | enhanced production of periplasmic interferon alpha-2b by escherichia coli using ion-exchange resin for in situ removal of acetate in the culture |
| url | http://psasir.upm.edu.my/id/eprint/22327/ http://psasir.upm.edu.my/id/eprint/22327/ http://psasir.upm.edu.my/id/eprint/22327/1/Enhanced%20production%20of%20periplasmic%20interferon%20alpha.pdf |