Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria
Macrobrachium rosenbergii nodavirus (MrNv) infects giant freshwater prawns and causes white tail disease (WTD). The coding region of the capsid protein of MrNv was amplified with RT-PCR and cloned into the pTrcHis2-TOPO vector. The recombinant plasmid was introduced into Escherichia coli and protein...
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| Format: | Article |
| Language: | English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22325/ http://psasir.upm.edu.my/id/eprint/22325/1/Virus-like%20particles%20of%20Macrobrachium%20rosenbergii%20nodavirus%20produced%20in%20bacteria.pdf |
| _version_ | 1848844455124140032 |
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| author | Zee, Hong Goh Tan, Soon Guan Bhassu, Subha Tan, Wen Siang |
| author_facet | Zee, Hong Goh Tan, Soon Guan Bhassu, Subha Tan, Wen Siang |
| author_sort | Zee, Hong Goh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Macrobrachium rosenbergii nodavirus (MrNv) infects giant freshwater prawns and causes white tail disease (WTD). The coding region of the capsid protein of MrNv was amplified with RT-PCR and cloned into the pTrcHis2-TOPO vector. The recombinant plasmid was introduced into Escherichia coli and protein expression was induced with IPTG. SDS-PAGE showed that the recombinant protein containing the His-tag and myc epitope has a molecular mass of about 46kDa and it was detected by the anti-His antibody in Western blotting. The protein was purified using immobilized metal affinity chromatography (IMAC) and transmission electron microscopic analysis revealed that the recombinant protein assembled into VLPs. |
| first_indexed | 2025-11-15T08:31:11Z |
| format | Article |
| id | upm-22325 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:11Z |
| publishDate | 2011 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223252015-11-23T02:58:22Z http://psasir.upm.edu.my/id/eprint/22325/ Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria Zee, Hong Goh Tan, Soon Guan Bhassu, Subha Tan, Wen Siang Macrobrachium rosenbergii nodavirus (MrNv) infects giant freshwater prawns and causes white tail disease (WTD). The coding region of the capsid protein of MrNv was amplified with RT-PCR and cloned into the pTrcHis2-TOPO vector. The recombinant plasmid was introduced into Escherichia coli and protein expression was induced with IPTG. SDS-PAGE showed that the recombinant protein containing the His-tag and myc epitope has a molecular mass of about 46kDa and it was detected by the anti-His antibody in Western blotting. The protein was purified using immobilized metal affinity chromatography (IMAC) and transmission electron microscopic analysis revealed that the recombinant protein assembled into VLPs. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22325/1/Virus-like%20particles%20of%20Macrobrachium%20rosenbergii%20nodavirus%20produced%20in%20bacteria.pdf Zee, Hong Goh and Tan, Soon Guan and Bhassu, Subha and Tan, Wen Siang (2011) Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria. Journal of Virological Methods, 175 (1). pp. 74-79. ISSN 0166-0934 10.1016/j.jviromet.2011.04.021 |
| spellingShingle | Zee, Hong Goh Tan, Soon Guan Bhassu, Subha Tan, Wen Siang Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria |
| title | Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria |
| title_full | Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria |
| title_fullStr | Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria |
| title_full_unstemmed | Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria |
| title_short | Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria |
| title_sort | virus-like particles of macrobrachium rosenbergii nodavirus produced in bacteria |
| url | http://psasir.upm.edu.my/id/eprint/22325/ http://psasir.upm.edu.my/id/eprint/22325/ http://psasir.upm.edu.my/id/eprint/22325/1/Virus-like%20particles%20of%20Macrobrachium%20rosenbergii%20nodavirus%20produced%20in%20bacteria.pdf |