Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids

This study examined the genetic characteristics of twenty-six microsatellite primers developed from three cyprinid fishes(Cyprinus carpio Linnaeus,Barbus barbus Linnaeus and Barbonymus gonionotus Bleeker) in two indigenous mahseer. The Tor douronensis Valenciennes were randomly collected from two l...

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Main Authors: Esa, Yuzine, Siraj, Siti Shapor, Abdul Rahim, Khairul Adha, Daud, Siti Khalijah, Ho, Ghim Chong, Tan, Soon Guan, Ismail, Mohammad Fadhil Syukri
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22302/
http://psasir.upm.edu.my/id/eprint/22302/1/Genetic%20characterization%20of%20two%20mahseer%20species.pdf
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author Esa, Yuzine
Siraj, Siti Shapor
Abdul Rahim, Khairul Adha
Daud, Siti Khalijah
Ho, Ghim Chong
Tan, Soon Guan
Ismail, Mohammad Fadhil Syukri
author_facet Esa, Yuzine
Siraj, Siti Shapor
Abdul Rahim, Khairul Adha
Daud, Siti Khalijah
Ho, Ghim Chong
Tan, Soon Guan
Ismail, Mohammad Fadhil Syukri
author_sort Esa, Yuzine
building UPM Institutional Repository
collection Online Access
description This study examined the genetic characteristics of twenty-six microsatellite primers developed from three cyprinid fishes(Cyprinus carpio Linnaeus,Barbus barbus Linnaeus and Barbonymus gonionotus Bleeker) in two indigenous mahseer. The Tor douronensis Valenciennes were randomly collected from two locations in Sarawak (N=52), while Tor tambroides Bleeker were obtained from Peninsular Malaysia (N=56). A total of ten and twelve primers were successfully amplified producing four and five polymorphic loci in T. douronensis and T. tambroides, respectively. The number of alleles perlocus ranging from 2 to 5 in T.douronensis and 2 to 7 in T. tambroides. A significant deviation from Hardy-Weinberg equilibrium (HWE) was observed at three loci (Barb37, Barb59 and Barb62) in one or more populations in T. tambroides while two loci (Barb37 and Barb62) were deviated in T. douronensis population of Batang Ai. Population structure analysis showed low level of inter-population genetic differentiation in both mahseer. Overall, the identified microsatellite loci should be useful in analysing T. douronensis and T. tambroides natural populations.
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institution Universiti Putra Malaysia
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language English
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spelling upm-223022018-10-17T04:31:22Z http://psasir.upm.edu.my/id/eprint/22302/ Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids Esa, Yuzine Siraj, Siti Shapor Abdul Rahim, Khairul Adha Daud, Siti Khalijah Ho, Ghim Chong Tan, Soon Guan Ismail, Mohammad Fadhil Syukri This study examined the genetic characteristics of twenty-six microsatellite primers developed from three cyprinid fishes(Cyprinus carpio Linnaeus,Barbus barbus Linnaeus and Barbonymus gonionotus Bleeker) in two indigenous mahseer. The Tor douronensis Valenciennes were randomly collected from two locations in Sarawak (N=52), while Tor tambroides Bleeker were obtained from Peninsular Malaysia (N=56). A total of ten and twelve primers were successfully amplified producing four and five polymorphic loci in T. douronensis and T. tambroides, respectively. The number of alleles perlocus ranging from 2 to 5 in T.douronensis and 2 to 7 in T. tambroides. A significant deviation from Hardy-Weinberg equilibrium (HWE) was observed at three loci (Barb37, Barb59 and Barb62) in one or more populations in T. tambroides while two loci (Barb37 and Barb62) were deviated in T. douronensis population of Batang Ai. Population structure analysis showed low level of inter-population genetic differentiation in both mahseer. Overall, the identified microsatellite loci should be useful in analysing T. douronensis and T. tambroides natural populations. Penerbit Universiti Kebangsaan Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22302/1/Genetic%20characterization%20of%20two%20mahseer%20species.pdf Esa, Yuzine and Siraj, Siti Shapor and Abdul Rahim, Khairul Adha and Daud, Siti Khalijah and Ho, Ghim Chong and Tan, Soon Guan and Ismail, Mohammad Fadhil Syukri (2011) Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids. Sains Malaysiana, 40 (10). pp. 1087-1095. ISSN 0026-6039 http://www.ukm.my/jsm/english_journals/vol40num10_2011/contentsVol40num10_2011.html
spellingShingle Esa, Yuzine
Siraj, Siti Shapor
Abdul Rahim, Khairul Adha
Daud, Siti Khalijah
Ho, Ghim Chong
Tan, Soon Guan
Ismail, Mohammad Fadhil Syukri
Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids
title Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids
title_full Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids
title_fullStr Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids
title_full_unstemmed Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids
title_short Genetic characterization of two mahseer species (Tor douronensis and Tor tambroides) using microsatellite markers from other cyprinids
title_sort genetic characterization of two mahseer species (tor douronensis and tor tambroides) using microsatellite markers from other cyprinids
url http://psasir.upm.edu.my/id/eprint/22302/
http://psasir.upm.edu.my/id/eprint/22302/
http://psasir.upm.edu.my/id/eprint/22302/1/Genetic%20characterization%20of%20two%20mahseer%20species.pdf