Production of long helical capsid of Nipah virus by Pichia pastoris.
The nucleocapsid (N) protein of Nipah virus (NiV) produced in a recombinant host can replace the use of inactivated virus as a diagnostic reagent because it is safer and affordable. The aim of this study was to express the N protein in Pichia pastoris. The N gene of NiV was cloned into the yeast exp...
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| Format: | Article |
| Language: | English English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22301/ http://psasir.upm.edu.my/id/eprint/22301/1/Production%20of%20long%20helical%20capsid%20of%20Nipah%20virus%20by%20Pichia%20pastoris.pdf |
| _version_ | 1848844449101119488 |
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| author | Joseph, Narcisse M. S. Tey, Beng Ti Tan, Chon Seng Shafee, Norazizah Tan, Wen Siang |
| author_facet | Joseph, Narcisse M. S. Tey, Beng Ti Tan, Chon Seng Shafee, Norazizah Tan, Wen Siang |
| author_sort | Joseph, Narcisse M. S. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The nucleocapsid (N) protein of Nipah virus (NiV) produced in a recombinant host can replace the use of inactivated virus as a diagnostic reagent because it is safer and affordable. The aim of this study was to express the N protein in Pichia pastoris. The N gene of NiV was cloned into the yeast expression vector, pPICZ B and expressed in P. pastoris. The recombinant N protein of NiV was purified using sucrose density gradient ultracentrifugation and was confirmed with Western blotting using rabbit anti-N antibody. The P. pastoris expressed N protein self-assembled into helical structures as large as 1.5 μm as shown in an electron micrograph. ELISA analysis performed with the swine sera obtained during the viral outbreak proved that the recombinant N protein to be highly antigenic. The NiV N protein produced in P. pastoris serves as an alternative to the recombinant N protein produced in Escherichia coli. |
| first_indexed | 2025-11-15T08:31:06Z |
| format | Article |
| id | upm-22301 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:31:06Z |
| publishDate | 2011 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223012015-10-06T02:12:12Z http://psasir.upm.edu.my/id/eprint/22301/ Production of long helical capsid of Nipah virus by Pichia pastoris. Joseph, Narcisse M. S. Tey, Beng Ti Tan, Chon Seng Shafee, Norazizah Tan, Wen Siang The nucleocapsid (N) protein of Nipah virus (NiV) produced in a recombinant host can replace the use of inactivated virus as a diagnostic reagent because it is safer and affordable. The aim of this study was to express the N protein in Pichia pastoris. The N gene of NiV was cloned into the yeast expression vector, pPICZ B and expressed in P. pastoris. The recombinant N protein of NiV was purified using sucrose density gradient ultracentrifugation and was confirmed with Western blotting using rabbit anti-N antibody. The P. pastoris expressed N protein self-assembled into helical structures as large as 1.5 μm as shown in an electron micrograph. ELISA analysis performed with the swine sera obtained during the viral outbreak proved that the recombinant N protein to be highly antigenic. The NiV N protein produced in P. pastoris serves as an alternative to the recombinant N protein produced in Escherichia coli. Elsevier 2011-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22301/1/Production%20of%20long%20helical%20capsid%20of%20Nipah%20virus%20by%20Pichia%20pastoris.pdf Joseph, Narcisse M. S. and Tey, Beng Ti and Tan, Chon Seng and Shafee, Norazizah and Tan, Wen Siang (2011) Production of long helical capsid of Nipah virus by Pichia pastoris. Process Biochemistry, 46 (9). pp. 1871-1874. ISSN 1359-5113; ESSN: 1873-3298 10.1016/j.procbio.2011.06.004 English |
| spellingShingle | Joseph, Narcisse M. S. Tey, Beng Ti Tan, Chon Seng Shafee, Norazizah Tan, Wen Siang Production of long helical capsid of Nipah virus by Pichia pastoris. |
| title | Production of long helical capsid of Nipah virus by Pichia pastoris. |
| title_full | Production of long helical capsid of Nipah virus by Pichia pastoris. |
| title_fullStr | Production of long helical capsid of Nipah virus by Pichia pastoris. |
| title_full_unstemmed | Production of long helical capsid of Nipah virus by Pichia pastoris. |
| title_short | Production of long helical capsid of Nipah virus by Pichia pastoris. |
| title_sort | production of long helical capsid of nipah virus by pichia pastoris. |
| url | http://psasir.upm.edu.my/id/eprint/22301/ http://psasir.upm.edu.my/id/eprint/22301/ http://psasir.upm.edu.my/id/eprint/22301/1/Production%20of%20long%20helical%20capsid%20of%20Nipah%20virus%20by%20Pichia%20pastoris.pdf |