High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry

Engkabang fat esters were produced via alcoholysis reaction between Engkabang fat and oleyl alcohol, catalyzed by Lipozyme RM IM. The reaction was carried out in a 500 ml Stirred tank reactor using heptane and hexane as solvents. Response surface methodology (RSM) based on a four-factor-five-level C...

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Main Authors: Abd. Rahman, Nur Fariza, Basri, Mahiran, Abdul Rahman, Mohd Basyaruddin, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar
Format: Article
Language:English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22293/
http://psasir.upm.edu.my/id/eprint/22293/1/High%20yield%20lipase.pdf
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author Abd. Rahman, Nur Fariza
Basri, Mahiran
Abdul Rahman, Mohd Basyaruddin
Raja Abdul Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
author_facet Abd. Rahman, Nur Fariza
Basri, Mahiran
Abdul Rahman, Mohd Basyaruddin
Raja Abdul Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
author_sort Abd. Rahman, Nur Fariza
building UPM Institutional Repository
collection Online Access
description Engkabang fat esters were produced via alcoholysis reaction between Engkabang fat and oleyl alcohol, catalyzed by Lipozyme RM IM. The reaction was carried out in a 500 ml Stirred tank reactor using heptane and hexane as solvents. Response surface methodology (RSM) based on a four-factor-five-level Central composite design (CCD) was applied to evaluate the effects of synthesis parameters, namely temperature, substrate molar ratio (oleyl alcohol: Engkabang fat), enzyme amount and impeller speed. The optimum yields of 96.2% and 91.4% were obtained for heptane and hexane at the optimum temperature of 53.9 °C, impeller speeds of 309.5 and 309.0 rpm, enzyme amounts of 4.82 and 5.65 g and substrate molar ratios of 2.94 and 3.39:1, respectively. The actual yields obtained compared well with the predicted values of 100.0% and 91.5%, respectively. Meanwhile, the properties of the esters show that they are suitable to be used as ingredient for cosmetic applications.
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spelling upm-222932016-09-27T07:03:40Z http://psasir.upm.edu.my/id/eprint/22293/ High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry Abd. Rahman, Nur Fariza Basri, Mahiran Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Engkabang fat esters were produced via alcoholysis reaction between Engkabang fat and oleyl alcohol, catalyzed by Lipozyme RM IM. The reaction was carried out in a 500 ml Stirred tank reactor using heptane and hexane as solvents. Response surface methodology (RSM) based on a four-factor-five-level Central composite design (CCD) was applied to evaluate the effects of synthesis parameters, namely temperature, substrate molar ratio (oleyl alcohol: Engkabang fat), enzyme amount and impeller speed. The optimum yields of 96.2% and 91.4% were obtained for heptane and hexane at the optimum temperature of 53.9 °C, impeller speeds of 309.5 and 309.0 rpm, enzyme amounts of 4.82 and 5.65 g and substrate molar ratios of 2.94 and 3.39:1, respectively. The actual yields obtained compared well with the predicted values of 100.0% and 91.5%, respectively. Meanwhile, the properties of the esters show that they are suitable to be used as ingredient for cosmetic applications. Elsevier 2011-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22293/1/High%20yield%20lipase.pdf Abd. Rahman, Nur Fariza and Basri, Mahiran and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2011) High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry. Bioresource Technology, 102 (3). pp. 2168-2176. ISSN 0960-8524; ESSN: 1873-2976 10.1016/j.biortech.2010.10.034
spellingShingle Abd. Rahman, Nur Fariza
Basri, Mahiran
Abdul Rahman, Mohd Basyaruddin
Raja Abdul Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry
title High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry
title_full High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry
title_fullStr High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry
title_full_unstemmed High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry
title_short High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry
title_sort high yield lipase-catalyzed synthesis of engkabang fat esters for the cosmetic industry
url http://psasir.upm.edu.my/id/eprint/22293/
http://psasir.upm.edu.my/id/eprint/22293/
http://psasir.upm.edu.my/id/eprint/22293/1/High%20yield%20lipase.pdf