High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM

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High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM

The mature ARM lipase gene was cloned into the pTrcHis expression vector and over-expressed in Escherichia coli TOP10 host. The optimum lipase expression was obtained after 18h post induction incubation with 1.0mM IPTG, where the lipase activity was approximately 1623-fold higher than wild type. A r...

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Bibliographic Details
Main Authors:	Ebrahimpour, Afshin, Raja Abdul Rahman, Raja Noor Zaliha, Basri, Mahiran, Salleh, Abu Bakar
Format:	Article
Language:	English
Published:	Elsevier 2011
Online Access:	http://psasir.upm.edu.my/id/eprint/22282/ http://psasir.upm.edu.my/id/eprint/22282/1/High%20level%20expression%20and%20characterization%20of%20a%20novel%20thermostable.pdf

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