Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator.
An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for mo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Springer Verlag
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22280/ http://psasir.upm.edu.my/id/eprint/22280/1/Electrochemical%20DNA%20biosensor%20for%20the%20detection%20of%20trichoderma%20harzianum%20based%20on%20agold%20electrode%20modified%20with%20a%20composite%20membrane%20made%20from%20an%20ionic%20liquid.pdf |
| _version_ | 1848844442843217920 |
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| author | Siddiquee, Shafiquzzaman Yusof, Nor Azah Salleh, Abu Bakar Tan, Soon Guan Abu Bakar, Fatimah |
| author_facet | Siddiquee, Shafiquzzaman Yusof, Nor Azah Salleh, Abu Bakar Tan, Soon Guan Abu Bakar, Fatimah |
| author_sort | Siddiquee, Shafiquzzaman |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for monitoring the hybridization of the target DNA. The biosensor was capable of detecting target DNA in the concentration range from 1.0 × 10-14 to 1.8 × 10-4 mol L-1, with a detection limit of 1.0 × 10-15 mol L-1. The approach towards constructing a DNA biosensor allows studies on the hybridization even with crude DNA fragments and also to analyze sample obtained from real samples. The results show that the DNA biosensor has the potential for sensitive detection of a specific sequence of the Trichoderma harzianum gene and provides a quick, sensitive and convenient method for the study of microorganisms. |
| first_indexed | 2025-11-15T08:31:00Z |
| format | Article |
| id | upm-22280 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:31:00Z |
| publishDate | 2011 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-222802016-02-18T04:37:59Z http://psasir.upm.edu.my/id/eprint/22280/ Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. Siddiquee, Shafiquzzaman Yusof, Nor Azah Salleh, Abu Bakar Tan, Soon Guan Abu Bakar, Fatimah An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for monitoring the hybridization of the target DNA. The biosensor was capable of detecting target DNA in the concentration range from 1.0 × 10-14 to 1.8 × 10-4 mol L-1, with a detection limit of 1.0 × 10-15 mol L-1. The approach towards constructing a DNA biosensor allows studies on the hybridization even with crude DNA fragments and also to analyze sample obtained from real samples. The results show that the DNA biosensor has the potential for sensitive detection of a specific sequence of the Trichoderma harzianum gene and provides a quick, sensitive and convenient method for the study of microorganisms. Springer Verlag 2011-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22280/1/Electrochemical%20DNA%20biosensor%20for%20the%20detection%20of%20trichoderma%20harzianum%20based%20on%20agold%20electrode%20modified%20with%20a%20composite%20membrane%20made%20from%20an%20ionic%20liquid.pdf Siddiquee, Shafiquzzaman and Yusof, Nor Azah and Salleh, Abu Bakar and Tan, Soon Guan and Abu Bakar, Fatimah (2011) Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. Microchimica Acta, 172 (3-4). pp. 357-363. ISSN 0026-3672; ESSN:1436-5073 http://link.springer.com/ 10.1007/s00604-010-0498-7 English |
| spellingShingle | Siddiquee, Shafiquzzaman Yusof, Nor Azah Salleh, Abu Bakar Tan, Soon Guan Abu Bakar, Fatimah Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. |
| title | Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. |
| title_full | Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. |
| title_fullStr | Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. |
| title_full_unstemmed | Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. |
| title_short | Electrochemical DNA biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator. |
| title_sort | electrochemical dna biosensor for the detection of trichoderma harzianum based on agold electrode modified with a composite membrane made from an ionic liquid, zno nanoparticles and chitosan, and by using acridine orange as a redox indicator. |
| url | http://psasir.upm.edu.my/id/eprint/22280/ http://psasir.upm.edu.my/id/eprint/22280/ http://psasir.upm.edu.my/id/eprint/22280/ http://psasir.upm.edu.my/id/eprint/22280/1/Electrochemical%20DNA%20biosensor%20for%20the%20detection%20of%20trichoderma%20harzianum%20based%20on%20agold%20electrode%20modified%20with%20a%20composite%20membrane%20made%20from%20an%20ionic%20liquid.pdf |