Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer.
The aims of this study were to isolate and characterize putative fragrance-related cDNAs from the floral cDNA library of Vanda Mimi Palmer, an orchid hybrid that has won several international awards for its sweet fragrance. A total of 1,000,000 pfu were screened by hybridizing cDNA library plaques w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Springer Verlag
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22264/ http://psasir.upm.edu.my/id/eprint/22264/1/Screening.pdf |
| _version_ | 1848844438277718016 |
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| author | Mohd-Hairul, Ab. Rahim Namasivayam, Parameswari Ong , Janna Abdullah Ee, Gwendoline Cheng Lian |
| author_facet | Mohd-Hairul, Ab. Rahim Namasivayam, Parameswari Ong , Janna Abdullah Ee, Gwendoline Cheng Lian |
| author_sort | Mohd-Hairul, Ab. Rahim |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The aims of this study were to isolate and characterize putative fragrance-related cDNAs from the floral cDNA library of Vanda Mimi Palmer, an orchid hybrid that has won several international awards for its sweet fragrance. A total of 1,000,000 pfu were screened by hybridizing cDNA library plaques with fully open flower cDNA probe of Vanda Mimi Palmer representing all mRNAs expressed during daytime. The clones that gave positive signals were in vivo excised and PCR-amplified inserts were subjected to reverse-Northern analysis by hybridizing with cDNA probes of fully open flower of Vanda Mimi Palmer (fragrant orchid) and its bud or fully open flower of Vanda Tan Chay Yan (non-fragrant orchid) separately. The clones up-regulated in fully open flower stage of Vanda Mimi Palmer compared to its bud stage or fully open flower of Vanda Tan Chay Yan were sequenced. Sequence analyses showed the presence of eight putative fragrance-related cDNAs of which two were putative Vanda Mimi Palmer 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol kinase (VMPCMEK) and Vanda Mimi Palmer cytochrome P450 protein (VMPCyP450). These two transcripts were selected for full-length cDNA isolation and expression analysis by real-time RT-PCR. The VMPCMEK transcript encodes a polypeptide of 400 amino acid residues, while the VMPCyP450 encodes 538 amino acid residues. Relative expression analysis of VMPCMEK and VMPCyP450 transcripts by real-time RT-PCR showed up-regulated expressions in floral tissues compared to vegetative tissues, and both were found to be developmentally regulated. |
| first_indexed | 2025-11-15T08:30:55Z |
| format | Article |
| id | upm-22264 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:30:55Z |
| publishDate | 2011 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-222642015-10-20T03:48:02Z http://psasir.upm.edu.my/id/eprint/22264/ Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer. Mohd-Hairul, Ab. Rahim Namasivayam, Parameswari Ong , Janna Abdullah Ee, Gwendoline Cheng Lian The aims of this study were to isolate and characterize putative fragrance-related cDNAs from the floral cDNA library of Vanda Mimi Palmer, an orchid hybrid that has won several international awards for its sweet fragrance. A total of 1,000,000 pfu were screened by hybridizing cDNA library plaques with fully open flower cDNA probe of Vanda Mimi Palmer representing all mRNAs expressed during daytime. The clones that gave positive signals were in vivo excised and PCR-amplified inserts were subjected to reverse-Northern analysis by hybridizing with cDNA probes of fully open flower of Vanda Mimi Palmer (fragrant orchid) and its bud or fully open flower of Vanda Tan Chay Yan (non-fragrant orchid) separately. The clones up-regulated in fully open flower stage of Vanda Mimi Palmer compared to its bud stage or fully open flower of Vanda Tan Chay Yan were sequenced. Sequence analyses showed the presence of eight putative fragrance-related cDNAs of which two were putative Vanda Mimi Palmer 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol kinase (VMPCMEK) and Vanda Mimi Palmer cytochrome P450 protein (VMPCyP450). These two transcripts were selected for full-length cDNA isolation and expression analysis by real-time RT-PCR. The VMPCMEK transcript encodes a polypeptide of 400 amino acid residues, while the VMPCyP450 encodes 538 amino acid residues. Relative expression analysis of VMPCMEK and VMPCyP450 transcripts by real-time RT-PCR showed up-regulated expressions in floral tissues compared to vegetative tissues, and both were found to be developmentally regulated. Springer Verlag 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22264/1/Screening.pdf Mohd-Hairul, Ab. Rahim and Namasivayam, Parameswari and Ong , Janna Abdullah and Ee, Gwendoline Cheng Lian (2011) Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer. Acta Physiologiae Plantarum, 33 (5). pp. 1651-1660. ISSN 0137-5881; ESSN:1861-1664 http://link.springer.com/ 10.1007/s11738-010-0701-9 English |
| spellingShingle | Mohd-Hairul, Ab. Rahim Namasivayam, Parameswari Ong , Janna Abdullah Ee, Gwendoline Cheng Lian Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer. |
| title | Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer. |
| title_full | Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer. |
| title_fullStr | Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer. |
| title_full_unstemmed | Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer. |
| title_short | Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer. |
| title_sort | screening, isolation, and molecular characterization of putative fragrance-related transcripts from vanda mimi palmer. |
| url | http://psasir.upm.edu.my/id/eprint/22264/ http://psasir.upm.edu.my/id/eprint/22264/ http://psasir.upm.edu.my/id/eprint/22264/ http://psasir.upm.edu.my/id/eprint/22264/1/Screening.pdf |