A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis).

Molecular markers developed for Prunus also offer a powerful tool to study the evolution of the genome, and for understanding of genome structure and determinants of genetic diversity. Two hundred eighty almond genotypes/cultivars from different origins distributed throughout Iran besides some forei...

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Main Authors: Kadkhodaei, S., Elahy, M., Nekouei, M. Khayyam, Imani, A., Shahnazari, M., Mardi, M., Javanmard, A., Ariff, Arbakariya
Format: Article
Language:English
English
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/22262/
http://psasir.upm.edu.my/id/eprint/22262/1/A%20panel%20of%20cultivate%20specific%20marker%20based%20on%20polymorphisms%20at%20microsatellite%20markers%20for%20Iranian%20cultivated%20Almonds.pdf
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author Kadkhodaei, S.
Elahy, M.
Nekouei, M. Khayyam
Imani, A.
Shahnazari, M.
Mardi, M.
Javanmard, A.
Ariff, Arbakariya
author_facet Kadkhodaei, S.
Elahy, M.
Nekouei, M. Khayyam
Imani, A.
Shahnazari, M.
Mardi, M.
Javanmard, A.
Ariff, Arbakariya
author_sort Kadkhodaei, S.
building UPM Institutional Repository
collection Online Access
description Molecular markers developed for Prunus also offer a powerful tool to study the evolution of the genome, and for understanding of genome structure and determinants of genetic diversity. Two hundred eighty almond genotypes/cultivars from different origins distributed throughout Iran besides some foreign cultivars and their hybrids with Iranian ones were collected. Microsatellite analysis was carried out using 9 pair flanking SSR sequences previously cloned and sequenced specifically in almond. The total number of detected alleles was 152 (9 to 20 alleles per locus with an average of 16.87). The mean PIC value of the polymorphic loci wasrelatively high (0.81) and the mean value for He was 0.83, so that we were able to distinguish 98% of the genotypes using 5 loci. Incluster analysis, the genotypes were divided into 2 major groups, foreign cultivars and Iranian almond genotypes. Principal coordinate analysis based on Shared Allele method indicated proper distribution of the studied markers through the genome. Some specific markers were recorded among the germ plasm which can be used efficiently in rapid and precise identification of the related genotypes and also in breeding programs through MAS. Genotypes were coded using our suggested coding method for genotype molecular identification.
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institution Universiti Putra Malaysia
institution_category Local University
language English
English
last_indexed 2025-11-15T08:30:55Z
publishDate 2010
recordtype eprints
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spelling upm-222622015-09-28T00:34:05Z http://psasir.upm.edu.my/id/eprint/22262/ A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis). Kadkhodaei, S. Elahy, M. Nekouei, M. Khayyam Imani, A. Shahnazari, M. Mardi, M. Javanmard, A. Ariff, Arbakariya Molecular markers developed for Prunus also offer a powerful tool to study the evolution of the genome, and for understanding of genome structure and determinants of genetic diversity. Two hundred eighty almond genotypes/cultivars from different origins distributed throughout Iran besides some foreign cultivars and their hybrids with Iranian ones were collected. Microsatellite analysis was carried out using 9 pair flanking SSR sequences previously cloned and sequenced specifically in almond. The total number of detected alleles was 152 (9 to 20 alleles per locus with an average of 16.87). The mean PIC value of the polymorphic loci wasrelatively high (0.81) and the mean value for He was 0.83, so that we were able to distinguish 98% of the genotypes using 5 loci. Incluster analysis, the genotypes were divided into 2 major groups, foreign cultivars and Iranian almond genotypes. Principal coordinate analysis based on Shared Allele method indicated proper distribution of the studied markers through the genome. Some specific markers were recorded among the germ plasm which can be used efficiently in rapid and precise identification of the related genotypes and also in breeding programs through MAS. Genotypes were coded using our suggested coding method for genotype molecular identification. 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22262/1/A%20panel%20of%20cultivate%20specific%20marker%20based%20on%20polymorphisms%20at%20microsatellite%20markers%20for%20Iranian%20cultivated%20Almonds.pdf Kadkhodaei, S. and Elahy, M. and Nekouei, M. Khayyam and Imani, A. and Shahnazari, M. and Mardi, M. and Javanmard, A. and Ariff, Arbakariya (2010) A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis). Australian Journal of Crop Science, 4 (9). pp. 730-736. ISSN 1835-2693 English
spellingShingle Kadkhodaei, S.
Elahy, M.
Nekouei, M. Khayyam
Imani, A.
Shahnazari, M.
Mardi, M.
Javanmard, A.
Ariff, Arbakariya
A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis).
title A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis).
title_full A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis).
title_fullStr A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis).
title_full_unstemmed A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis).
title_short A panel of cultivate specific marker based on polymorphisms at microsatellite markers for Iranian cultivated Almonds (Prunus dulcis).
title_sort panel of cultivate specific marker based on polymorphisms at microsatellite markers for iranian cultivated almonds (prunus dulcis).
url http://psasir.upm.edu.my/id/eprint/22262/
http://psasir.upm.edu.my/id/eprint/22262/1/A%20panel%20of%20cultivate%20specific%20marker%20based%20on%20polymorphisms%20at%20microsatellite%20markers%20for%20Iranian%20cultivated%20Almonds.pdf