Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication
Species-specific polymerase chain reaction (PCR) analysis of a conserved region in the mitochondrial (mt) 12S ribosomal RNA (rRNA) gene was developed for species identification from raw pork and lard samples. Genomic DNA of pork and lard were successfully extracted and were found to be of good quali...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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IDOSI Publications
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22247/ http://psasir.upm.edu.my/id/eprint/22247/1/Specific%20polymerase%20chain%20reaction.pdf |
| _version_ | 1848844433533960192 |
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| author | Azmi, Aida Azrina Che Man, Yaakob Al-Kahtani, Hassan A. Abdul Rahim, Raha Radu, Son |
| author_facet | Azmi, Aida Azrina Che Man, Yaakob Al-Kahtani, Hassan A. Abdul Rahim, Raha Radu, Son |
| author_sort | Azmi, Aida Azrina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Species-specific polymerase chain reaction (PCR) analysis of a conserved region in the mitochondrial (mt) 12S ribosomal RNA (rRNA) gene was developed for species identification from raw pork and lard samples. Genomic DNA of pork and lard were successfully extracted and were found to be of good quality. The extracted genomic DNA was then subjected to PCR amplification targeting the specific regions of the 12S rRNA gene and produced clear PCR products on the amplification of 12S rRNA gene of 387 base pairs (bp) from pig species. The species-specific PCR identification yielded excellent results for identification of pig. This made it deal for quality control purposes and a potentially reliable technique to avoid species adulteration for Halal authentication and verification. |
| first_indexed | 2025-11-15T08:30:51Z |
| format | Article |
| id | upm-22247 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:30:51Z |
| publishDate | 2011 |
| publisher | IDOSI Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-222472015-10-21T03:16:55Z http://psasir.upm.edu.my/id/eprint/22247/ Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication Azmi, Aida Azrina Che Man, Yaakob Al-Kahtani, Hassan A. Abdul Rahim, Raha Radu, Son Species-specific polymerase chain reaction (PCR) analysis of a conserved region in the mitochondrial (mt) 12S ribosomal RNA (rRNA) gene was developed for species identification from raw pork and lard samples. Genomic DNA of pork and lard were successfully extracted and were found to be of good quality. The extracted genomic DNA was then subjected to PCR amplification targeting the specific regions of the 12S rRNA gene and produced clear PCR products on the amplification of 12S rRNA gene of 387 base pairs (bp) from pig species. The species-specific PCR identification yielded excellent results for identification of pig. This made it deal for quality control purposes and a potentially reliable technique to avoid species adulteration for Halal authentication and verification. IDOSI Publications 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22247/1/Specific%20polymerase%20chain%20reaction.pdf Azmi, Aida Azrina and Che Man, Yaakob and Al-Kahtani, Hassan A. and Abdul Rahim, Raha and Radu, Son (2011) Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication. Middle-East Journal of Scientific Research, 7 (6). pp. 1008-1013. ISSN 1990-9233 http://www.idosi.org/mejsr/mejsr7(6)11.htm |
| spellingShingle | Azmi, Aida Azrina Che Man, Yaakob Al-Kahtani, Hassan A. Abdul Rahim, Raha Radu, Son Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication |
| title | Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication |
| title_full | Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication |
| title_fullStr | Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication |
| title_full_unstemmed | Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication |
| title_short | Specific polymerase chain reaction (PCR) analysis of raw meats and fats of pigs for Halal authentication |
| title_sort | specific polymerase chain reaction (pcr) analysis of raw meats and fats of pigs for halal authentication |
| url | http://psasir.upm.edu.my/id/eprint/22247/ http://psasir.upm.edu.my/id/eprint/22247/ http://psasir.upm.edu.my/id/eprint/22247/1/Specific%20polymerase%20chain%20reaction.pdf |