Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man.
White tail disease (WTD) is a serious viral disease in the hatcheries and nursery ponds of Macrobrachium rosenbergii in many parts of the world. A new disease similar to WTD was observed in larvae and post larvae of M.rosenbergii cultured in Malaysia. In the present study,RT-PCR assay was used to...
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| Format: | Article |
| Language: | English English |
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Springer
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22234/ http://psasir.upm.edu.my/id/eprint/22234/1/Detection%20and%20phylogenetic%20profiling%20of%20nodavirus%20associated%20with%20white%20tail%20disease%20in%20Malaysian%20Macrobrachium%20rosenbergii%20de%20Man.pdf |
| _version_ | 1848844429842972672 |
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| author | Saedi, Tayebeh Azam Moeni, Hassan Tan, Wen Siang Yusoff, Khatijah Mohd. Daud, Hassan Chu, Kua Beng Tan, Soon Guan Bhassu, Subha |
| author_facet | Saedi, Tayebeh Azam Moeni, Hassan Tan, Wen Siang Yusoff, Khatijah Mohd. Daud, Hassan Chu, Kua Beng Tan, Soon Guan Bhassu, Subha |
| author_sort | Saedi, Tayebeh Azam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | White tail disease (WTD) is a serious viral disease in the hatcheries and nursery ponds of Macrobrachium rosenbergii
in many parts of the world. A new disease similar to WTD was observed in larvae and post larvae of M.rosenbergii
cultured in Malaysia. In the present study,RT-PCR assay was used to detect the causative agents of WTD, M.rosenbergii
nodavirus (MrNV) and extra small virus (XSV) using specific primers for MrNV RNA2 and XSV. The results showed the presence of MrNV in the samples with or without signs of WTD. However, XSV was only detected in some of the MrNV-positive samples.Phylogenetic analysis showed that the RNA2 of our Malaysian isolates were significantly different from the other isolates. Histopathological studies revealed myofiber degeneration of the tail muscles and liquefactive myopathy in the infected prawns. This was the first report on the occurrence of MrNV in the Malaysian freshwater prawn. |
| first_indexed | 2025-11-15T08:30:47Z |
| format | Article |
| id | upm-22234 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:30:47Z |
| publishDate | 2012 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-222342016-02-17T02:59:04Z http://psasir.upm.edu.my/id/eprint/22234/ Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. Saedi, Tayebeh Azam Moeni, Hassan Tan, Wen Siang Yusoff, Khatijah Mohd. Daud, Hassan Chu, Kua Beng Tan, Soon Guan Bhassu, Subha White tail disease (WTD) is a serious viral disease in the hatcheries and nursery ponds of Macrobrachium rosenbergii in many parts of the world. A new disease similar to WTD was observed in larvae and post larvae of M.rosenbergii cultured in Malaysia. In the present study,RT-PCR assay was used to detect the causative agents of WTD, M.rosenbergii nodavirus (MrNV) and extra small virus (XSV) using specific primers for MrNV RNA2 and XSV. The results showed the presence of MrNV in the samples with or without signs of WTD. However, XSV was only detected in some of the MrNV-positive samples.Phylogenetic analysis showed that the RNA2 of our Malaysian isolates were significantly different from the other isolates. Histopathological studies revealed myofiber degeneration of the tail muscles and liquefactive myopathy in the infected prawns. This was the first report on the occurrence of MrNV in the Malaysian freshwater prawn. Springer 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22234/1/Detection%20and%20phylogenetic%20profiling%20of%20nodavirus%20associated%20with%20white%20tail%20disease%20in%20Malaysian%20Macrobrachium%20rosenbergii%20de%20Man.pdf Saedi, Tayebeh Azam and Moeni, Hassan and Tan, Wen Siang and Yusoff, Khatijah and Mohd. Daud, Hassan and Chu, Kua Beng and Tan, Soon Guan and Bhassu, Subha (2012) Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. Molecular Biology Reports, 39 (5). pp. 5785-5790. ISSN 0301-4851; ESSN: 1573-4978 http://link.springer.com/journal/11033/39/5/page/5 10.1007/s11033-011-1389-7 English |
| spellingShingle | Saedi, Tayebeh Azam Moeni, Hassan Tan, Wen Siang Yusoff, Khatijah Mohd. Daud, Hassan Chu, Kua Beng Tan, Soon Guan Bhassu, Subha Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. |
| title | Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. |
| title_full | Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. |
| title_fullStr | Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. |
| title_full_unstemmed | Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. |
| title_short | Detection and phylogenetic profiling of nodavirus associated with white tail disease in Malaysian Macrobrachium rosenbergii de Man. |
| title_sort | detection and phylogenetic profiling of nodavirus associated with white tail disease in malaysian macrobrachium rosenbergii de man. |
| url | http://psasir.upm.edu.my/id/eprint/22234/ http://psasir.upm.edu.my/id/eprint/22234/ http://psasir.upm.edu.my/id/eprint/22234/ http://psasir.upm.edu.my/id/eprint/22234/1/Detection%20and%20phylogenetic%20profiling%20of%20nodavirus%20associated%20with%20white%20tail%20disease%20in%20Malaysian%20Macrobrachium%20rosenbergii%20de%20Man.pdf |