Molybdate reduction by Pseudomonas sp. strain DRY2.

Aims:To isolate and characterize a potent molybdenum-reducing bacterium.Methods and Results:A minimal salt medium supplemented with 10 mmol l)1molybdate, glucose (1Æ0%, w⁄v) as a carbon source and ammonium sulfate(0Æ3%, w⁄v) as a nitrogen source was used in the screening process. A molyb-denum-reduc...

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Main Authors:	Abd. Shukor, Mohd. Yunus, Ahmad, Siti Aqlima, Mohd Nadzir, Mas Muniroh, Abdullah, M. P., Shamaan, Nor Aripin, Syed, Mohd. Arif
Format:	Article
Language:	English English
Published:	The Society for Applied Microbiology 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/22229/ http://psasir.upm.edu.my/id/eprint/22229/1/Molybdate%20reduction%20by%20Pseudomonas%20sp.pdf

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author	Abd. Shukor, Mohd. Yunus Ahmad, Siti Aqlima Mohd Nadzir, Mas Muniroh Abdullah, M. P. Shamaan, Nor Aripin Syed, Mohd. Arif
author_facet	Abd. Shukor, Mohd. Yunus Ahmad, Siti Aqlima Mohd Nadzir, Mas Muniroh Abdullah, M. P. Shamaan, Nor Aripin Syed, Mohd. Arif
author_sort	Abd. Shukor, Mohd. Yunus
building	UPM Institutional Repository
collection	Online Access
description	Aims:To isolate and characterize a potent molybdenum-reducing bacterium.Methods and Results:A minimal salt medium supplemented with 10 mmol l)1molybdate, glucose (1Æ0%, w⁄v) as a carbon source and ammonium sulfate(0Æ3%, w⁄v) as a nitrogen source was used in the screening process. A molyb-denum-reducing bacterium was isolated and tentatively identified as Pseudomo-nassp. strain DRY2 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. Strain DRY2 produced 2 Æ4,3Æ2 and 6Æ 2 times more molybdenum blue compared to Serratia marcescens strain DRY6,Enterobacter cloacae strain 48 and Eschericia coliK12, respectively.Molybdate reduction as optimum at 5 mmol l)1phosphate. The optimum molybdate concentration that supported molybdate reduction at 5 mmol l)1phosphate was between 15 and 25 mmol l)1. Molybdate reduction was optimum at 40C and at pH 6Æ0. Phosphate concentrations higher than 5 mmol l)1 strongly inhibited molybdate reduction. Inhibitors of electron transport system such as antimycin A, rotenone, sodium azide and cyanide did not inhibit the molybdenum-reducing enzyme activity. Chromium, copper, mercury and lead inhibited the molybdenum-reducing activity.Conclusions:A novel molybdenum-reducing bacterium with high molybdenum reduction capacity has been isolated. Significance and Impact of the Study:Molybdenum is an emerging global pollutant that is very toxic to ruminants. The characteristics of this bacterium suggest that it would be useful in the bioremediation of molybdenum pollutant.
first_indexed	2025-11-15T08:30:46Z
format	Article
id	upm-22229
institution	Universiti Putra Malaysia
institution_category	Local University
language	English English
last_indexed	2025-11-15T08:30:46Z
publishDate	2010
publisher	The Society for Applied Microbiology
recordtype	eprints
repository_type	Digital Repository
spelling	upm-222292015-11-02T08:40:56Z http://psasir.upm.edu.my/id/eprint/22229/ Molybdate reduction by Pseudomonas sp. strain DRY2. Abd. Shukor, Mohd. Yunus Ahmad, Siti Aqlima Mohd Nadzir, Mas Muniroh Abdullah, M. P. Shamaan, Nor Aripin Syed, Mohd. Arif Aims:To isolate and characterize a potent molybdenum-reducing bacterium.Methods and Results:A minimal salt medium supplemented with 10 mmol l)1molybdate, glucose (1Æ0%, w⁄v) as a carbon source and ammonium sulfate(0Æ3%, w⁄v) as a nitrogen source was used in the screening process. A molyb-denum-reducing bacterium was isolated and tentatively identified as Pseudomo-nassp. strain DRY2 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. Strain DRY2 produced 2 Æ4,3Æ2 and 6Æ 2 times more molybdenum blue compared to Serratia marcescens strain DRY6,Enterobacter cloacae strain 48 and Eschericia coliK12, respectively.Molybdate reduction as optimum at 5 mmol l)1phosphate. The optimum molybdate concentration that supported molybdate reduction at 5 mmol l)1phosphate was between 15 and 25 mmol l)1. Molybdate reduction was optimum at 40C and at pH 6Æ0. Phosphate concentrations higher than 5 mmol l)1 strongly inhibited molybdate reduction. Inhibitors of electron transport system such as antimycin A, rotenone, sodium azide and cyanide did not inhibit the molybdenum-reducing enzyme activity. Chromium, copper, mercury and lead inhibited the molybdenum-reducing activity.Conclusions:A novel molybdenum-reducing bacterium with high molybdenum reduction capacity has been isolated. Significance and Impact of the Study:Molybdenum is an emerging global pollutant that is very toxic to ruminants. The characteristics of this bacterium suggest that it would be useful in the bioremediation of molybdenum pollutant. The Society for Applied Microbiology 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22229/1/Molybdate%20reduction%20by%20Pseudomonas%20sp.pdf Abd. Shukor, Mohd. Yunus and Ahmad, Siti Aqlima and Mohd Nadzir, Mas Muniroh and Abdullah, M. P. and Shamaan, Nor Aripin and Syed, Mohd. Arif (2010) Molybdate reduction by Pseudomonas sp. strain DRY2. Journal of Applied Microbiology, 108 (6). pp. 2050-2058. ISSN 1364-5072 10.1111/j.1365-2672.2009.04604.x English
spellingShingle	Abd. Shukor, Mohd. Yunus Ahmad, Siti Aqlima Mohd Nadzir, Mas Muniroh Abdullah, M. P. Shamaan, Nor Aripin Syed, Mohd. Arif Molybdate reduction by Pseudomonas sp. strain DRY2.
title	Molybdate reduction by Pseudomonas sp. strain DRY2.
title_full	Molybdate reduction by Pseudomonas sp. strain DRY2.
title_fullStr	Molybdate reduction by Pseudomonas sp. strain DRY2.
title_full_unstemmed	Molybdate reduction by Pseudomonas sp. strain DRY2.
title_short	Molybdate reduction by Pseudomonas sp. strain DRY2.
title_sort	molybdate reduction by pseudomonas sp. strain dry2.
url	http://psasir.upm.edu.my/id/eprint/22229/ http://psasir.upm.edu.my/id/eprint/22229/ http://psasir.upm.edu.my/id/eprint/22229/1/Molybdate%20reduction%20by%20Pseudomonas%20sp.pdf

Molybdate reduction by Pseudomonas sp. strain DRY2.

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