Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; fol...
| Main Author: | |
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| Format: | Article |
| Language: | English English |
| Published: |
1983
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| Online Access: | http://psasir.upm.edu.my/id/eprint/2221/ http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf |
| _version_ | 1848839186328584192 |
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| author | Mahmood, Marziah |
| author_facet | Mahmood, Marziah |
| author_sort | Mahmood, Marziah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus
angustifolius could be partially or fully restored by the addition of divalent cations to the incubation
mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed
by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2
+ (49%). The Mn-deficient enzyme was more sensitive to
manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme.
However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient
enzyme achieved a maximum activity of 105% of control at 2.0mM. |
| first_indexed | 2025-11-15T07:07:27Z |
| format | Article |
| id | upm-2221 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:07:27Z |
| publishDate | 1983 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-22212015-04-13T04:27:37Z http://psasir.upm.edu.my/id/eprint/2221/ Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. Mahmood, Marziah The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2 + (49%). The Mn-deficient enzyme was more sensitive to manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme. However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient enzyme achieved a maximum activity of 105% of control at 2.0mM. 1983 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf Mahmood, Marziah (1983) Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. Pertanika, 6 (1). pp. 63-66. English |
| spellingShingle | Mahmood, Marziah Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
| title | Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
| title_full | Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
| title_fullStr | Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
| title_full_unstemmed | Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
| title_short | Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
| title_sort | properties of glutamate dehydrogenase in developing legume fruit. ii.the effects of divalent cations. |
| url | http://psasir.upm.edu.my/id/eprint/2221/ http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf |