Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus

Catharanthus roseus var. rosea, infected with Malaysian isolate of cucumber mosaic virus (CMV-MP), exhibited leaf mosaic, leaf deformation and malformed flowers. Electron microscopic examination of the infected leaf cells revealed significant alteration of the chloroplasts in the mesophyll cells. La...

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Main Authors: Mamat, Mazidah, Lau, Wei Hong, Yusoff, Khatijah, Hashim, Habibuddin, Tan, Yee How
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2012
Online Access:http://psasir.upm.edu.my/id/eprint/20949/
http://psasir.upm.edu.my/id/eprint/20949/1/85.%20Ultrastructural%20Features%20of%20Catharanthus%20roseus%20Leaves%20Infected.pdf
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author Mamat, Mazidah
Lau, Wei Hong
Yusoff, Khatijah
Hashim, Habibuddin
Tan, Yee How
author_facet Mamat, Mazidah
Lau, Wei Hong
Yusoff, Khatijah
Hashim, Habibuddin
Tan, Yee How
author_sort Mamat, Mazidah
building UPM Institutional Repository
collection Online Access
description Catharanthus roseus var. rosea, infected with Malaysian isolate of cucumber mosaic virus (CMV-MP), exhibited leaf mosaic, leaf deformation and malformed flowers. Electron microscopic examination of the infected leaf cells revealed significant alteration of the chloroplasts in the mesophyll cells. Large starch grains in necrotic zones and disorganized thylakoid system were the most prominent modifications observed within the chloroplasts of the infected tissues. Meanwhile, membrane-bound vesicles were detected in the vacuoles of the CMV-MP-infected leaf cells. A crystalline array of phytoferritin macromolecules was detected in the chloroplast at 40 days post-inoculation. However, neither single nor aggregate of CMV-MP particles was detected in the cytoplasm due to difficulties in differentiating them from the ribosomes. Nonetheless, structure resembling the inclusion bodies, commonly produced after virus infection, could not be found in the infected leaf cells. Similarly, structure abnormality in the nucleus or mitochondria was also not observed.
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spelling upm-209492015-11-24T07:11:18Z http://psasir.upm.edu.my/id/eprint/20949/ Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus Mamat, Mazidah Lau, Wei Hong Yusoff, Khatijah Hashim, Habibuddin Tan, Yee How Catharanthus roseus var. rosea, infected with Malaysian isolate of cucumber mosaic virus (CMV-MP), exhibited leaf mosaic, leaf deformation and malformed flowers. Electron microscopic examination of the infected leaf cells revealed significant alteration of the chloroplasts in the mesophyll cells. Large starch grains in necrotic zones and disorganized thylakoid system were the most prominent modifications observed within the chloroplasts of the infected tissues. Meanwhile, membrane-bound vesicles were detected in the vacuoles of the CMV-MP-infected leaf cells. A crystalline array of phytoferritin macromolecules was detected in the chloroplast at 40 days post-inoculation. However, neither single nor aggregate of CMV-MP particles was detected in the cytoplasm due to difficulties in differentiating them from the ribosomes. Nonetheless, structure resembling the inclusion bodies, commonly produced after virus infection, could not be found in the infected leaf cells. Similarly, structure abnormality in the nucleus or mitochondria was also not observed. Universiti Putra Malaysia Press 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/20949/1/85.%20Ultrastructural%20Features%20of%20Catharanthus%20roseus%20Leaves%20Infected.pdf Mamat, Mazidah and Lau, Wei Hong and Yusoff, Khatijah and Hashim, Habibuddin and Tan, Yee How (2012) Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus. Pertanika Journal of Tropical Agricultural Science, 35 (S). pp. 85-92. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(S)%20Dec.%202012/09_page%2085-92.pdf
spellingShingle Mamat, Mazidah
Lau, Wei Hong
Yusoff, Khatijah
Hashim, Habibuddin
Tan, Yee How
Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus
title Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus
title_full Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus
title_fullStr Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus
title_full_unstemmed Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus
title_short Ultrastructural features of Catharanthus roseus leaves infected with cucumber mosaic virus
title_sort ultrastructural features of catharanthus roseus leaves infected with cucumber mosaic virus
url http://psasir.upm.edu.my/id/eprint/20949/
http://psasir.upm.edu.my/id/eprint/20949/
http://psasir.upm.edu.my/id/eprint/20949/1/85.%20Ultrastructural%20Features%20of%20Catharanthus%20roseus%20Leaves%20Infected.pdf