Detection of Newcastle disease virus.
A method for detection of Newcastle disease virus in a biological sample comprising (a) the extraction of mRNA from the sample (b) converting the mRNA from step (a) with reverse transcriptase under conditions allowing for the production of cDNA; ? amplifying the cDNA from step (b) by polymerase chai...
| Main Authors: | , , , |
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| Format: | Patent |
| Published: |
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/20884/ |
| _version_ | 1848844083066306560 |
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| author | Yusoff, Khatijah Kho, Chiew Ling Mohd Lila, Mohd Azmi Arshad, Siti Suri |
| author_facet | Yusoff, Khatijah Kho, Chiew Ling Mohd Lila, Mohd Azmi Arshad, Siti Suri |
| author_sort | Yusoff, Khatijah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A method for detection of Newcastle disease virus in a biological sample comprising (a) the extraction of mRNA from the sample (b) converting the mRNA from step (a) with reverse transcriptase under conditions allowing for the production of cDNA; ? amplifying the cDNA from step (b) by polymerase chain reaction to form an amplified PCR product (d) converting the amplified PCR product from step ? with a pair of nested primers to form a nested labelled PCR streptavidin-coated microtiter plate; (f) adding a peroxidase substrate to the immobilised product from step (e) to produce a coloured reaction product; and (g) detecting the resulting coloured reaction product using an ELISA reader.
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| first_indexed | 2025-11-15T08:25:17Z |
| format | Patent |
| id | upm-20884 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T08:25:17Z |
| publishDate | 2007 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-208842013-06-14T02:50:27Z http://psasir.upm.edu.my/id/eprint/20884/ Detection of Newcastle disease virus. Yusoff, Khatijah Kho, Chiew Ling Mohd Lila, Mohd Azmi Arshad, Siti Suri A method for detection of Newcastle disease virus in a biological sample comprising (a) the extraction of mRNA from the sample (b) converting the mRNA from step (a) with reverse transcriptase under conditions allowing for the production of cDNA; ? amplifying the cDNA from step (b) by polymerase chain reaction to form an amplified PCR product (d) converting the amplified PCR product from step ? with a pair of nested primers to form a nested labelled PCR streptavidin-coated microtiter plate; (f) adding a peroxidase substrate to the immobilised product from step (e) to produce a coloured reaction product; and (g) detecting the resulting coloured reaction product using an ELISA reader. 2007-12-31 Patent NonPeerReviewed Khatijah Yusoff (2007) Detection of Newcastle disease virus. UNSPECIFIED. |
| spellingShingle | Yusoff, Khatijah Kho, Chiew Ling Mohd Lila, Mohd Azmi Arshad, Siti Suri Detection of Newcastle disease virus. |
| title | Detection of Newcastle disease virus.
|
| title_full | Detection of Newcastle disease virus.
|
| title_fullStr | Detection of Newcastle disease virus.
|
| title_full_unstemmed | Detection of Newcastle disease virus.
|
| title_short | Detection of Newcastle disease virus.
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| title_sort | detection of newcastle disease virus. |
| url | http://psasir.upm.edu.my/id/eprint/20884/ |