Enzyme and microbial technology: biotechnology of fats and oils.
To develop an enzyme technology industry, a sizeable enzyme bank is a priority requirement. A group of enzymes of interest to us and currently attracting attention of researchers throughout the world are the lipases. Apart from their usual hydrolytic properties, these enzymes can perform syntheti...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
1991
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/20856/ http://psasir.upm.edu.my/id/eprint/20856/1/ID%2020856.pdf |
| _version_ | 1848844076678381568 |
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| author | Salleh, Abu Bakar Abd. Razak, Che Nyonya Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran |
| author_facet | Salleh, Abu Bakar Abd. Razak, Che Nyonya Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran |
| author_sort | Salleh, Abu Bakar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | To develop an enzyme technology industry, a sizeable enzyme bank is a priority
requirement. A group of enzymes of interest to us and currently attracting
attention of researchers throughout the world are the lipases. Apart from
their usual hydrolytic properties, these enzymes can perform synthetic
reactions under controlled conditions. Our group have meticulously carried
out a screening programme for microbes that can produce these enzymes. So far
we have isolated over 50 microbial isolates exhibiting lipolytic activities
on plate and managed to study in detail 3 fungi and 2 bacteria. To improve
the versatility of these enzymes we are redesigning the enzymes with the hope
of obtaining an analogue with better solubility in organic solvents. We have
covalently attached aldehydes of various sizes and hydrophobicity onto the
enzyme lipase, and the enzyme thus derivatised showed. enhanced catalytic
activity and thermal stability compared with the native unmodified enzyme.
Another approach is to immobilise the enzyme. The enzyme molecule is still, in principle, to another molecule. In most cases, the molecule attached yo the enzyme is usually insoluble, hence confering the derivatised enzyme a new property of insolubility. We have shown that enzymes can be
immobilised by simple adsorption onto readily available resins. |
| first_indexed | 2025-11-15T08:25:10Z |
| format | Conference or Workshop Item |
| id | upm-20856 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:25:10Z |
| publishDate | 1991 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-208562014-08-26T08:50:40Z http://psasir.upm.edu.my/id/eprint/20856/ Enzyme and microbial technology: biotechnology of fats and oils. Salleh, Abu Bakar Abd. Razak, Che Nyonya Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran To develop an enzyme technology industry, a sizeable enzyme bank is a priority requirement. A group of enzymes of interest to us and currently attracting attention of researchers throughout the world are the lipases. Apart from their usual hydrolytic properties, these enzymes can perform synthetic reactions under controlled conditions. Our group have meticulously carried out a screening programme for microbes that can produce these enzymes. So far we have isolated over 50 microbial isolates exhibiting lipolytic activities on plate and managed to study in detail 3 fungi and 2 bacteria. To improve the versatility of these enzymes we are redesigning the enzymes with the hope of obtaining an analogue with better solubility in organic solvents. We have covalently attached aldehydes of various sizes and hydrophobicity onto the enzyme lipase, and the enzyme thus derivatised showed. enhanced catalytic activity and thermal stability compared with the native unmodified enzyme. Another approach is to immobilise the enzyme. The enzyme molecule is still, in principle, to another molecule. In most cases, the molecule attached yo the enzyme is usually insoluble, hence confering the derivatised enzyme a new property of insolubility. We have shown that enzymes can be immobilised by simple adsorption onto readily available resins. 1991 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/20856/1/ID%2020856.pdf Salleh, Abu Bakar and Abd. Razak, Che Nyonya and Wan Yunus, Wan Md. Zin and Ampon, Kamaruzaman and Basri, Mahiran (1991) Enzyme and microbial technology: biotechnology of fats and oils. In: Seminar Kebangsaan Menilai Pencapaian Penyelidikan IRPA Dalam Rancangan Malaysia ke-5 Sektor Perindustrian (20-24 Disember 1991) hosted in Universiti Utara Malaysia, Kedah, 20-24 Disember 1991, Universiti Utara Malaysia, Kedah. . Enzymes - Immobilized enzymes Oils and fats - Biotechnology Microbial biotechnology English |
| spellingShingle | Enzymes - Immobilized enzymes Oils and fats - Biotechnology Microbial biotechnology Salleh, Abu Bakar Abd. Razak, Che Nyonya Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran Enzyme and microbial technology: biotechnology of fats and oils. |
| title | Enzyme and microbial technology: biotechnology of fats and oils. |
| title_full | Enzyme and microbial technology: biotechnology of fats and oils. |
| title_fullStr | Enzyme and microbial technology: biotechnology of fats and oils. |
| title_full_unstemmed | Enzyme and microbial technology: biotechnology of fats and oils. |
| title_short | Enzyme and microbial technology: biotechnology of fats and oils. |
| title_sort | enzyme and microbial technology: biotechnology of fats and oils. |
| topic | Enzymes - Immobilized enzymes Oils and fats - Biotechnology Microbial biotechnology |
| url | http://psasir.upm.edu.my/id/eprint/20856/ http://psasir.upm.edu.my/id/eprint/20856/1/ID%2020856.pdf |