Novel lipase gene from Bacillus sphaericus 205y
A biologically pure culture of Bacillus sphaericus strain 205Y capable of producing an organic solvent - tolerant lipase was isolated from soil samples via direct plating method using 1 (V/V) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source, ethyl benzene and...
| Main Authors: | , , , |
|---|---|
| Format: | Patent |
| Published: |
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/20420/ |
| _version_ | 1848843974944489472 |
|---|---|
| author | Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Chin, John Hun |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Chin, John Hun |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A biologically pure culture of Bacillus sphaericus strain 205Y capable of producing an organic solvent - tolerant lipase was isolated from soil samples via direct plating method using 1 (V/V) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source, ethyl benzene and P-xylene. The lipase gene was isolated via genomic library and sequenced. The production of lipase gene from Bacillus sphaericus strain 205Y is a novel gene. The lipase gene was cloned from Bacillus spaericus strain 205Y and the recombinant lipase was efficiently excreted into the culture medium using expression vectors bearing the lipase gene. |
| first_indexed | 2025-11-15T08:23:33Z |
| format | Patent |
| id | upm-20420 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T08:23:33Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-204202016-09-27T01:44:28Z http://psasir.upm.edu.my/id/eprint/20420/ Novel lipase gene from Bacillus sphaericus 205y Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Chin, John Hun A biologically pure culture of Bacillus sphaericus strain 205Y capable of producing an organic solvent - tolerant lipase was isolated from soil samples via direct plating method using 1 (V/V) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source, ethyl benzene and P-xylene. The lipase gene was isolated via genomic library and sequenced. The production of lipase gene from Bacillus sphaericus strain 205Y is a novel gene. The lipase gene was cloned from Bacillus spaericus strain 205Y and the recombinant lipase was efficiently excreted into the culture medium using expression vectors bearing the lipase gene. 2009-10-30 Patent NonPeerReviewed Raja Noor Zaliha Raja Abd. Rahman (2009) Novel lipase gene from Bacillus sphaericus 205y. PI20040958. |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Chin, John Hun Novel lipase gene from Bacillus sphaericus 205y |
| title | Novel lipase gene from Bacillus sphaericus 205y |
| title_full | Novel lipase gene from Bacillus sphaericus 205y |
| title_fullStr | Novel lipase gene from Bacillus sphaericus 205y |
| title_full_unstemmed | Novel lipase gene from Bacillus sphaericus 205y |
| title_short | Novel lipase gene from Bacillus sphaericus 205y |
| title_sort | novel lipase gene from bacillus sphaericus 205y |
| url | http://psasir.upm.edu.my/id/eprint/20420/ |