Identification of pork in raw meat using porcine-specific polymerase chain reaction assay
The porcine-specific polymerase chain reaction (PCR) assay was developed to detect the presence of pork in raw meat either in pure or in a mixture with different meat species. A porcine-specific DNA fragment could be amplified using porcine-specific primer designed based on a porcine specific sequen...
| Main Authors: | , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Halal Products Research Institute, Universiti Putra Malaysia
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/19938/ http://psasir.upm.edu.my/id/eprint/19938/1/73-14.pdf |
| _version_ | 1848843860092911616 |
|---|---|
| author | Khairil Mokhtar, Nur Fadhilah Mustafa, Shuhaimi Sazili, Awis Qurni Nordin, Rumaisa Che Man, Yaakob |
| author_facet | Khairil Mokhtar, Nur Fadhilah Mustafa, Shuhaimi Sazili, Awis Qurni Nordin, Rumaisa Che Man, Yaakob |
| author_sort | Khairil Mokhtar, Nur Fadhilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The porcine-specific polymerase chain reaction (PCR) assay was developed to detect the presence of pork in raw meat either in pure or in a mixture with different meat species. A porcine-specific DNA fragment could be amplified using porcine-specific primer designed based on a porcine specific sequence of mitochondrial D-loop gene. Amplification of porcine DNA produced 174 bp porcine-specific DNA fragment and no PCR products detected when assay applied to DNA of other meat species confirming the specificity of the primers. The assay has a high potential to be employed in detection of pork in raw meat products. |
| first_indexed | 2025-11-15T08:21:44Z |
| format | Conference or Workshop Item |
| id | upm-19938 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:21:44Z |
| publishDate | 2009 |
| publisher | Halal Products Research Institute, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-199382018-08-03T08:36:01Z http://psasir.upm.edu.my/id/eprint/19938/ Identification of pork in raw meat using porcine-specific polymerase chain reaction assay Khairil Mokhtar, Nur Fadhilah Mustafa, Shuhaimi Sazili, Awis Qurni Nordin, Rumaisa Che Man, Yaakob The porcine-specific polymerase chain reaction (PCR) assay was developed to detect the presence of pork in raw meat either in pure or in a mixture with different meat species. A porcine-specific DNA fragment could be amplified using porcine-specific primer designed based on a porcine specific sequence of mitochondrial D-loop gene. Amplification of porcine DNA produced 174 bp porcine-specific DNA fragment and no PCR products detected when assay applied to DNA of other meat species confirming the specificity of the primers. The assay has a high potential to be employed in detection of pork in raw meat products. Halal Products Research Institute, Universiti Putra Malaysia 2009 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/19938/1/73-14.pdf Khairil Mokhtar, Nur Fadhilah and Mustafa, Shuhaimi and Sazili, Awis Qurni and Nordin, Rumaisa and Che Man, Yaakob (2009) Identification of pork in raw meat using porcine-specific polymerase chain reaction assay. In: 3rd IMT-GT International Symposium on Halal science and Management 2009, 21-22 Dec. 2009, KLIA Sepang, Malaysia. (pp. 101-104). |
| spellingShingle | Khairil Mokhtar, Nur Fadhilah Mustafa, Shuhaimi Sazili, Awis Qurni Nordin, Rumaisa Che Man, Yaakob Identification of pork in raw meat using porcine-specific polymerase chain reaction assay |
| title | Identification of pork in raw meat using porcine-specific polymerase chain reaction assay |
| title_full | Identification of pork in raw meat using porcine-specific polymerase chain reaction assay |
| title_fullStr | Identification of pork in raw meat using porcine-specific polymerase chain reaction assay |
| title_full_unstemmed | Identification of pork in raw meat using porcine-specific polymerase chain reaction assay |
| title_short | Identification of pork in raw meat using porcine-specific polymerase chain reaction assay |
| title_sort | identification of pork in raw meat using porcine-specific polymerase chain reaction assay |
| url | http://psasir.upm.edu.my/id/eprint/19938/ http://psasir.upm.edu.my/id/eprint/19938/1/73-14.pdf |