The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis
The presence of KODAK Photoflo-600 (Cat.No.4055786),which contains ethylene glycol,in the cellulose acetate electrophoresis (CAE) buffers enhances the activities and sharpens the bands formed by several goat and buffalo blood enzymes on gels. This solution also overcame the problems encountered with...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
1990
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| Online Access: | http://psasir.upm.edu.my/id/eprint/19363/ http://psasir.upm.edu.my/id/eprint/19363/1/19363.pdf |
| _version_ | 1848843706628571136 |
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| author | O. S., Selvaraj M., Sekaran J. M., Pandaran T. K., Mukherjee Tan, S. G. Barker, J. S. F. |
| author_facet | O. S., Selvaraj M., Sekaran J. M., Pandaran T. K., Mukherjee Tan, S. G. Barker, J. S. F. |
| author_sort | O. S., Selvaraj |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The presence of KODAK Photoflo-600 (Cat.No.4055786),which contains ethylene glycol,in the cellulose acetate electrophoresis (CAE) buffers enhances the activities and sharpens the bands formed by several goat and buffalo blood enzymes on gels. This solution also overcame the problems encountered with overheating in some buffer systems. Its presence in the gel buffer also made it easier to soak the gel strips without any entrapment of air bubles. Malic enzyme (ME),malate dehydrogenase (MDH). 6-Phosphoglucose dehydrogenase(6pgdh). Glucose phosphate isomerase (GPI). Isocitrate dehydrogenase (IDH) and NADH-Diaphorase (NADH-DIA) from both buffaloes and goats exhibited ,arked improvement in band resolution and staining properties. |
| first_indexed | 2025-11-15T08:19:18Z |
| format | Conference or Workshop Item |
| id | upm-19363 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:19:18Z |
| publishDate | 1990 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-193632015-05-14T08:32:03Z http://psasir.upm.edu.my/id/eprint/19363/ The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis O. S., Selvaraj M., Sekaran J. M., Pandaran T. K., Mukherjee Tan, S. G. Barker, J. S. F. The presence of KODAK Photoflo-600 (Cat.No.4055786),which contains ethylene glycol,in the cellulose acetate electrophoresis (CAE) buffers enhances the activities and sharpens the bands formed by several goat and buffalo blood enzymes on gels. This solution also overcame the problems encountered with overheating in some buffer systems. Its presence in the gel buffer also made it easier to soak the gel strips without any entrapment of air bubles. Malic enzyme (ME),malate dehydrogenase (MDH). 6-Phosphoglucose dehydrogenase(6pgdh). Glucose phosphate isomerase (GPI). Isocitrate dehydrogenase (IDH) and NADH-Diaphorase (NADH-DIA) from both buffaloes and goats exhibited ,arked improvement in band resolution and staining properties. 1990 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/19363/1/19363.pdf O. S., Selvaraj and M., Sekaran and J. M., Pandaran and T. K., Mukherjee and Tan, S. G. and Barker, J. S. F. (1990) The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis. In: 15th Malaysian Biochemical Society Conference, 3 - 4 Sept. 1990, Universiti Kebangsaan Malaysia, Bangi, Selangor. (pp. 166-169). (Unpublished) |
| spellingShingle | O. S., Selvaraj M., Sekaran J. M., Pandaran T. K., Mukherjee Tan, S. G. Barker, J. S. F. The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis |
| title | The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis |
| title_full | The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis |
| title_fullStr | The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis |
| title_full_unstemmed | The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis |
| title_short | The use of Kodak photoflo-600 solution in cellulose acetate electrophoresis |
| title_sort | use of kodak photoflo-600 solution in cellulose acetate electrophoresis |
| url | http://psasir.upm.edu.my/id/eprint/19363/ http://psasir.upm.edu.my/id/eprint/19363/1/19363.pdf |