Large scale dielectrophoretic construction of biofilms using textile technology
Arrays of microelectrodes for AC electrokinetic experiments were fabricated by weaving together stainless steel wires (weft) and flexible polyester yarn (warp) in a plain weave pattern. The cloth produced can be used to collect cells in low conductivity media by dielectrophoresis (DEP). The construc...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley Periodicals
2007
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/18726/ http://psasir.upm.edu.my/id/eprint/18726/1/Large%20scale%20dielectrophoretic%20construction%20of%20biofilms%20using%20textile%20technology.pdf |
| _version_ | 1848843577586614272 |
|---|---|
| author | Zainal Abidin, Zurina Downes, Les Markx, Gerard H. |
| author_facet | Zainal Abidin, Zurina Downes, Les Markx, Gerard H. |
| author_sort | Zainal Abidin, Zurina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Arrays of microelectrodes for AC electrokinetic experiments were fabricated by weaving together stainless steel wires (weft) and flexible polyester yarn (warp) in a plain weave pattern. The cloth produced can be used to collect cells in low conductivity media by dielectrophoresis (DEP). The construction of model biofilms consisting of a yeast layer on top of a layer of M. luteus is demonstrated, using polyethylenimine (PEI) as the flocculating agent. This technique offers an alternative to the formation of biofilms at microelectrodes made by photolithography, and would allow the construction of biofilms with defined internal architectures by DEP at much larger scales than was possible previously. Furthermore, the flexibility of the cloth would also allow it to be distorted or folded into various shapes. |
| first_indexed | 2025-11-15T08:17:14Z |
| format | Article |
| id | upm-18726 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:17:14Z |
| publishDate | 2007 |
| publisher | Wiley Periodicals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-187262015-11-26T04:00:52Z http://psasir.upm.edu.my/id/eprint/18726/ Large scale dielectrophoretic construction of biofilms using textile technology Zainal Abidin, Zurina Downes, Les Markx, Gerard H. Arrays of microelectrodes for AC electrokinetic experiments were fabricated by weaving together stainless steel wires (weft) and flexible polyester yarn (warp) in a plain weave pattern. The cloth produced can be used to collect cells in low conductivity media by dielectrophoresis (DEP). The construction of model biofilms consisting of a yeast layer on top of a layer of M. luteus is demonstrated, using polyethylenimine (PEI) as the flocculating agent. This technique offers an alternative to the formation of biofilms at microelectrodes made by photolithography, and would allow the construction of biofilms with defined internal architectures by DEP at much larger scales than was possible previously. Furthermore, the flexibility of the cloth would also allow it to be distorted or folded into various shapes. Wiley Periodicals 2007-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18726/1/Large%20scale%20dielectrophoretic%20construction%20of%20biofilms%20using%20textile%20technology.pdf Zainal Abidin, Zurina and Downes, Les and Markx, Gerard H. (2007) Large scale dielectrophoretic construction of biofilms using textile technology. Biotechnology and Bioengineering, 96 (6). pp. 1222-1225. ISSN 0006-3592; ESSN: 1097-0290 http://onlinelibrary.wiley.com/doi/10.1002/bit.21228/abstract 10.1002/bit.21228 |
| spellingShingle | Zainal Abidin, Zurina Downes, Les Markx, Gerard H. Large scale dielectrophoretic construction of biofilms using textile technology |
| title | Large scale dielectrophoretic construction of biofilms using textile technology |
| title_full | Large scale dielectrophoretic construction of biofilms using textile technology |
| title_fullStr | Large scale dielectrophoretic construction of biofilms using textile technology |
| title_full_unstemmed | Large scale dielectrophoretic construction of biofilms using textile technology |
| title_short | Large scale dielectrophoretic construction of biofilms using textile technology |
| title_sort | large scale dielectrophoretic construction of biofilms using textile technology |
| url | http://psasir.upm.edu.my/id/eprint/18726/ http://psasir.upm.edu.my/id/eprint/18726/ http://psasir.upm.edu.my/id/eprint/18726/ http://psasir.upm.edu.my/id/eprint/18726/1/Large%20scale%20dielectrophoretic%20construction%20of%20biofilms%20using%20textile%20technology.pdf |