Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus
Specific-pathogen-free (SPF) chickens infected with very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 developed lower pathogenicity compared to UPM97/61. Sequence analysis indicated that UPM94/273 is an exceptional vvIBDV. In this study, a SYBR® Green I based real-time reverse tran...
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Elsevier
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18407/ http://psasir.upm.edu.my/id/eprint/18407/1/Comparative%20analysis%20of%20viral%20RNA%20and%20apoptotic%20cells%20in%20bursae%20following%20infection%20with%20infectious%20bursal%20disease%20virus.pdf |
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| author | Kong, Lih Ling Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Seow, Heng Fong |
| author_facet | Kong, Lih Ling Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Seow, Heng Fong |
| author_sort | Kong, Lih Ling |
| building | UPM Institutional Repository |
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| description | Specific-pathogen-free (SPF) chickens infected with very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 developed lower pathogenicity compared to UPM97/61. Sequence analysis indicated that UPM94/273 is an exceptional vvIBDV. In this study, a SYBR® Green I based real-time reverse transcriptase reaction assay was developed to measure viral RNA in the bursae of SPF chickens infected with IBDV. Specificity of the amplified products was confirmed by melting temperature analysis. A linear relationship was observed between the amount of input viral RNA and the threshold values for IBDV-specific product over five log10 dilutions. The viral RNA level following infection with UPM94/273 was significantly higher at day 1 and 2 post-inoculation (p.i.) compared to UPM97/61 infected chickens. However, chickens infected with UPM97/61 had significantly higher numbers of bursal cells undergoing apoptosis compared to UPM94/273 infected chickens. In both groups, the number of apoptotic cells and viral RNA levels peak at day 3 p.i. This study indicates that UPM97/61 and UPM94/273 have different efficiency of replication and percentage of apoptotic cells in bursae during the acute phase of IBDV infection. © 2004 Elsevier Ltd. All rights reserved. |
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| format | Article |
| id | upm-18407 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:16:02Z |
| publishDate | 2004 |
| publisher | Elsevier |
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| spelling | upm-184072015-09-28T08:51:37Z http://psasir.upm.edu.my/id/eprint/18407/ Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus Kong, Lih Ling Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Seow, Heng Fong Specific-pathogen-free (SPF) chickens infected with very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 developed lower pathogenicity compared to UPM97/61. Sequence analysis indicated that UPM94/273 is an exceptional vvIBDV. In this study, a SYBR® Green I based real-time reverse transcriptase reaction assay was developed to measure viral RNA in the bursae of SPF chickens infected with IBDV. Specificity of the amplified products was confirmed by melting temperature analysis. A linear relationship was observed between the amount of input viral RNA and the threshold values for IBDV-specific product over five log10 dilutions. The viral RNA level following infection with UPM94/273 was significantly higher at day 1 and 2 post-inoculation (p.i.) compared to UPM97/61 infected chickens. However, chickens infected with UPM97/61 had significantly higher numbers of bursal cells undergoing apoptosis compared to UPM94/273 infected chickens. In both groups, the number of apoptotic cells and viral RNA levels peak at day 3 p.i. This study indicates that UPM97/61 and UPM94/273 have different efficiency of replication and percentage of apoptotic cells in bursae during the acute phase of IBDV infection. © 2004 Elsevier Ltd. All rights reserved. Elsevier 2004-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18407/1/Comparative%20analysis%20of%20viral%20RNA%20and%20apoptotic%20cells%20in%20bursae%20following%20infection%20with%20infectious%20bursal%20disease%20virus.pdf Kong, Lih Ling and Omar, Abdul Rahman and Bejo, Mohd Hair and Ideris, Aini and Seow, Heng Fong (2004) Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus. Comparative Immunology, Microbiology and Infectious Diseases, 27 (6). pp. 433-443. ISSN 0147-9571 http://dx.doi.org/10.1016/j.cimid.2004.01.006 Apoptosis Birds - Virus diseases RNA Viruses - pathogenicity doi:10.1016/j.cimid.2004.01.006 English |
| spellingShingle | Apoptosis Birds - Virus diseases RNA Viruses - pathogenicity Kong, Lih Ling Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Seow, Heng Fong Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_full | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_fullStr | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_full_unstemmed | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_short | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_sort | comparative analysis of viral rna and apoptotic cells in bursae following infection with infectious bursal disease virus |
| topic | Apoptosis Birds - Virus diseases RNA Viruses - pathogenicity |
| url | http://psasir.upm.edu.my/id/eprint/18407/ http://psasir.upm.edu.my/id/eprint/18407/ http://psasir.upm.edu.my/id/eprint/18407/ http://psasir.upm.edu.my/id/eprint/18407/1/Comparative%20analysis%20of%20viral%20RNA%20and%20apoptotic%20cells%20in%20bursae%20following%20infection%20with%20infectious%20bursal%20disease%20virus.pdf |