Suppression of apoptosis in perfusion culture of Myeloma NS0 cells enhances cell growth but reduces antibody productivity

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Suppression of apoptosis in perfusion culture of Myeloma NS0 cells enhances cell growth but reduces antibody productivity

A spin filter perfusion systems was used to achieve a high cell density culture for two NS0 cell lines in 2 litres bioreactors. One cell line is transfected with the bcl-2 gene (NS0 Bcl-2) encodes the 'anti-apoptotic' human Bcl-2 protein and the other cell line (NS0 Control) with a blank v...

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Bibliographic Details
Main Authors:	Tey, Beng Ti, Al-Rubeai, Mohamed
Format:	Article
Language:	English English
Published:	Kluwer Academic Publishers 2004
Subjects:	Antibodies, Monoclonal Apoptosis Cell Culture Techniques
Online Access:	http://psasir.upm.edu.my/id/eprint/18402/ http://psasir.upm.edu.my/id/eprint/18402/1/Suppression%20of%20apoptosis%20in%20perfusion%20culture%20of%20Myeloma%20NS0%20cells%20enhances%20cell%20growth%20but%20reduces%20antibody%20productivity.pdf

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