Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones
Morinda elliptica (Rubiaceae) cell suspension cultures were established in shake flask system for the production of anthraquinones. The optimized medium formulation for cell growth and anthraquinone production is proposed. Murashige and Skoog's basal medium (MS) was found to be the best medium,...
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| Format: | Article |
| Language: | English English English |
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Springer
1998
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18379/ http://psasir.upm.edu.my/id/eprint/18379/1/Establishment%20of%20cell%20suspension%20cultures%20of%20Morinda%20elliptica%20for%20the%20production%20of%20anthraquinones.pdf http://psasir.upm.edu.my/id/eprint/18379/7/A_1006108717255.pdf |
| _version_ | 1848843495388741632 |
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| author | Abdullah, Mohd Azmuddin Ali, Abdul Manaf Mahmood, Maziah Lajis, Md Nordin Ariff, Arbakariya |
| author_facet | Abdullah, Mohd Azmuddin Ali, Abdul Manaf Mahmood, Maziah Lajis, Md Nordin Ariff, Arbakariya |
| author_sort | Abdullah, Mohd Azmuddin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Morinda elliptica (Rubiaceae) cell suspension cultures were established in shake flask system for the production of anthraquinones. The optimized medium formulation for cell growth and anthraquinone production is proposed. Murashige and Skoog's basal medium (MS) was found to be the best medium, used in combination with 0.5 mg l-1 NAA and 0.5 mg l-1 kinetin. At the range of sucrose concentration tested (3-8% w/v), 8% was the best in enhancing both cell growth and anthraquinone production. A strategy to formulate growth and production medium by manipulating culture age and inoculum age, the type of medium formulation used to grow inoculum, incubation temperature and light intensity was established. By using 18 month old culture and 7 day old inoculum at incubation temperature of 27 ± 3°C, anthraquinone yield of 2.9 g l-1 and 4.5 g l-1, under illumination of 1200 lux and in the dark was obtained, respectively. |
| first_indexed | 2025-11-15T08:15:56Z |
| format | Article |
| id | upm-18379 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-15T08:15:56Z |
| publishDate | 1998 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-183792024-08-05T02:45:22Z http://psasir.upm.edu.my/id/eprint/18379/ Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones Abdullah, Mohd Azmuddin Ali, Abdul Manaf Mahmood, Maziah Lajis, Md Nordin Ariff, Arbakariya Morinda elliptica (Rubiaceae) cell suspension cultures were established in shake flask system for the production of anthraquinones. The optimized medium formulation for cell growth and anthraquinone production is proposed. Murashige and Skoog's basal medium (MS) was found to be the best medium, used in combination with 0.5 mg l-1 NAA and 0.5 mg l-1 kinetin. At the range of sucrose concentration tested (3-8% w/v), 8% was the best in enhancing both cell growth and anthraquinone production. A strategy to formulate growth and production medium by manipulating culture age and inoculum age, the type of medium formulation used to grow inoculum, incubation temperature and light intensity was established. By using 18 month old culture and 7 day old inoculum at incubation temperature of 27 ± 3°C, anthraquinone yield of 2.9 g l-1 and 4.5 g l-1, under illumination of 1200 lux and in the dark was obtained, respectively. Springer 1998 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/18379/1/Establishment%20of%20cell%20suspension%20cultures%20of%20Morinda%20elliptica%20for%20the%20production%20of%20anthraquinones.pdf text en http://psasir.upm.edu.my/id/eprint/18379/7/A_1006108717255.pdf Abdullah, Mohd Azmuddin and Ali, Abdul Manaf and Mahmood, Maziah and Lajis, Md Nordin and Ariff, Arbakariya (1998) Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones. Plant Cell, Tissue and Organ Culture, 54 (3). pp. 173-182. ISSN 0167-6857 http://dx.doi.org/10.1023/A:1006108717255 Anthraquinones Morinda - Biotechnology - Malaysia Plant tissue culture doi:10.1023/A:1006108717255 English |
| spellingShingle | Anthraquinones Morinda - Biotechnology - Malaysia Plant tissue culture Abdullah, Mohd Azmuddin Ali, Abdul Manaf Mahmood, Maziah Lajis, Md Nordin Ariff, Arbakariya Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones |
| title | Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones |
| title_full | Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones |
| title_fullStr | Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones |
| title_full_unstemmed | Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones |
| title_short | Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones |
| title_sort | establishment of cell suspension cultures of morinda elliptica for the production of anthraquinones |
| topic | Anthraquinones Morinda - Biotechnology - Malaysia Plant tissue culture |
| url | http://psasir.upm.edu.my/id/eprint/18379/ http://psasir.upm.edu.my/id/eprint/18379/ http://psasir.upm.edu.my/id/eprint/18379/ http://psasir.upm.edu.my/id/eprint/18379/1/Establishment%20of%20cell%20suspension%20cultures%20of%20Morinda%20elliptica%20for%20the%20production%20of%20anthraquinones.pdf http://psasir.upm.edu.my/id/eprint/18379/7/A_1006108717255.pdf |