Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor
Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica (Rubiaceae) cell suspension cultures in a stirred-tank bioreactor are described. Of all the strategies applied, only bubble-free aeration was successful in eliminating foaming by 100%. Despite the foaming effe...
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| Format: | Article |
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Springer
2000
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18378/ http://psasir.upm.edu.my/id/eprint/18378/1/Strategies%20to%20overcome%20foaming%20and%20wall.pdf http://psasir.upm.edu.my/id/eprint/18378/7/A_1006495107778.pdf |
| _version_ | 1848843495093043200 |
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| author | Abdullah, Mohd Azmuddin Ariff, Arbakariya Mahmood, Maziah Ali, Abdul Manaf Lajis, Md Nordin |
| author_facet | Abdullah, Mohd Azmuddin Ariff, Arbakariya Mahmood, Maziah Ali, Abdul Manaf Lajis, Md Nordin |
| author_sort | Abdullah, Mohd Azmuddin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica (Rubiaceae) cell suspension cultures in a stirred-tank bioreactor are described. Of all the strategies applied, only bubble-free aeration was successful in eliminating foaming by 100%. Despite the foaming effect of around 40% in G medium strategy with 0.012% (v/v) antifoam, the maximum dry cell weight attained (19.2 g 1-1) and anthraquinone (AQ) content (4.0 mg g-1 DW) was nearly three times higher than that achieved in cultivation using 0.025% (v/v) antifoam. For continuous cell growth, the effect of inoculum age should also be considered when anti-foam is to be added. P medium strategy, without antifoam addition, not only promoted both growth (18 g 1-1) and AQ production (9.8 mg g-1 DW), but also resulted in lower foaming and wall-growth (below 30% level), and higher foaming reduction (30-40%). |
| first_indexed | 2025-11-15T08:15:56Z |
| format | Article |
| id | upm-18378 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-15T08:15:56Z |
| publishDate | 2000 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-183782024-08-05T07:13:01Z http://psasir.upm.edu.my/id/eprint/18378/ Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor Abdullah, Mohd Azmuddin Ariff, Arbakariya Mahmood, Maziah Ali, Abdul Manaf Lajis, Md Nordin Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica (Rubiaceae) cell suspension cultures in a stirred-tank bioreactor are described. Of all the strategies applied, only bubble-free aeration was successful in eliminating foaming by 100%. Despite the foaming effect of around 40% in G medium strategy with 0.012% (v/v) antifoam, the maximum dry cell weight attained (19.2 g 1-1) and anthraquinone (AQ) content (4.0 mg g-1 DW) was nearly three times higher than that achieved in cultivation using 0.025% (v/v) antifoam. For continuous cell growth, the effect of inoculum age should also be considered when anti-foam is to be added. P medium strategy, without antifoam addition, not only promoted both growth (18 g 1-1) and AQ production (9.8 mg g-1 DW), but also resulted in lower foaming and wall-growth (below 30% level), and higher foaming reduction (30-40%). Springer 2000 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/18378/1/Strategies%20to%20overcome%20foaming%20and%20wall.pdf text en http://psasir.upm.edu.my/id/eprint/18378/7/A_1006495107778.pdf Abdullah, Mohd Azmuddin and Ariff, Arbakariya and Mahmood, Maziah and Ali, Abdul Manaf and Lajis, Md Nordin (2000) Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor. Plant Cell, Tissue and Organ Culture, 60 (3). pp. 205-212. ISSN 0167-6857 http://dx.doi.org/10.1023/A:1006495107778 Morinda - Biotechnology - Malaysia Plant cell culture Anthraquinones doi:10.1023/A:1006495107778 English |
| spellingShingle | Morinda - Biotechnology - Malaysia Plant cell culture Anthraquinones Abdullah, Mohd Azmuddin Ariff, Arbakariya Mahmood, Maziah Ali, Abdul Manaf Lajis, Md Nordin Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor |
| title | Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor |
| title_full | Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor |
| title_fullStr | Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor |
| title_full_unstemmed | Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor |
| title_short | Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica cell suspension culture in a stirred-tank bioreactor |
| title_sort | strategies to overcome foaming and wall-growth during the cultivation of morinda elliptica cell suspension culture in a stirred-tank bioreactor |
| topic | Morinda - Biotechnology - Malaysia Plant cell culture Anthraquinones |
| url | http://psasir.upm.edu.my/id/eprint/18378/ http://psasir.upm.edu.my/id/eprint/18378/ http://psasir.upm.edu.my/id/eprint/18378/ http://psasir.upm.edu.my/id/eprint/18378/1/Strategies%20to%20overcome%20foaming%20and%20wall.pdf http://psasir.upm.edu.my/id/eprint/18378/7/A_1006495107778.pdf |