Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption

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Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption

Enzymatic methods have been used to cleave the C- or N-terminus polyhistidine tags from histidine tagged proteins following expanded bed purification using immobilized metal affinitychromatography (IMAC). This study assesses the use of Factor Xa and a genetically engineered exopeptidase dipeptidyl a...

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Bibliographic Details
Main Authors:	Abdullah, Norhafizah, Chase, Howard Allaker
Format:	Article
Language:	English
Published:	Wiley Periodicals 2005
Online Access:	http://psasir.upm.edu.my/id/eprint/18361/ http://psasir.upm.edu.my/id/eprint/18361/1/Removal%20of%20poly.pdf

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