Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption
Enzymatic methods have been used to cleave the C- or N-terminus polyhistidine tags from histidine tagged proteins following expanded bed purification using immobilized metal affinitychromatography (IMAC). This study assesses the use of Factor Xa and a genetically engineered exopeptidase dipeptidyl a...
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| Format: | Article |
| Language: | English |
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Wiley Periodicals
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18361/ http://psasir.upm.edu.my/id/eprint/18361/1/Removal%20of%20poly.pdf |
| _version_ | 1848843490510766080 |
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| author | Abdullah, Norhafizah Chase, Howard Allaker |
| author_facet | Abdullah, Norhafizah Chase, Howard Allaker |
| author_sort | Abdullah, Norhafizah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enzymatic methods have been used to cleave the C- or N-terminus polyhistidine tags from histidine tagged proteins following expanded bed purification using immobilized metal affinitychromatography (IMAC). This study assesses the use of Factor Xa and a genetically engineered exopeptidase dipeptidyl aminopeptidase-1 (DAPase-1)for the removal of C-terminusand N-terminus polyhistidine tags, respectively. Model proteins consisting of maltose binding protein (MBP) having a C- or N-terminal polyhistidine tag were used. Digestion of the hexahistidinetagofMBP-His6 by Factor Xa and HT 15-MBP by DAPase-1 was successful. The time taken to complete the conversion of MBP-HiS6 to MBP was 16 h, as judged by SDS-PAGE and Western blots against anti-His antibody. When the detagged protein was purified using subtractive IMAC, the yield was moderate at 71% although the overall recovery was high at 95%. Likewise, a yield of 79% and a recovery of 97% was obtained when digestion was performed with using "on-column" tag digestion. Oncolumn tag digestion involves cleavage of histidine tag from polyhistidine tagged proteins that are still bound to the IMAC column. Digestion of an N-terminal polyhistidine tag from HT15-MBP (1 mg/mL) by the DAPase-1 system was superiorto the results obtained with Factor Xa with a higher yield and recovery of 99% and 95%, respectively. The digestion by DAPase-1 system was faster and was complete at 5 h as opposed to 16 h for Factor Xa. The detagged MBP proteins were isolated from the digestion mixtures using a simple subtractive IMAC column procedure with the detagged protein appearing in the flowthrough and washing fractions while residual dipeptides and DAPase-1 (which was engineered to exhibit a poly-His tail) were adsorbed to the column. FPLC analysis using a MonoS cation exchanger was performed to understand and monitor the progress and time course of DAPase-1 digestion of HT15-MBP to MBP. Optimization of process variables such as temperature, protein concentration, and enzyme activity was developed for the DAPase-1 digesting system on HT15-MBP to MBP. In short, this study proved that the use of either Factor Xa or DAPase-l for the digestion of polyhistidine tags is simple and efficient and can be carried out under mild reaction conditions. |
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| format | Article |
| id | upm-18361 |
| institution | Universiti Putra Malaysia |
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| language | English |
| last_indexed | 2025-11-15T08:15:51Z |
| publishDate | 2005 |
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| spelling | upm-183612017-11-01T09:53:41Z http://psasir.upm.edu.my/id/eprint/18361/ Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption Abdullah, Norhafizah Chase, Howard Allaker Enzymatic methods have been used to cleave the C- or N-terminus polyhistidine tags from histidine tagged proteins following expanded bed purification using immobilized metal affinitychromatography (IMAC). This study assesses the use of Factor Xa and a genetically engineered exopeptidase dipeptidyl aminopeptidase-1 (DAPase-1)for the removal of C-terminusand N-terminus polyhistidine tags, respectively. Model proteins consisting of maltose binding protein (MBP) having a C- or N-terminal polyhistidine tag were used. Digestion of the hexahistidinetagofMBP-His6 by Factor Xa and HT 15-MBP by DAPase-1 was successful. The time taken to complete the conversion of MBP-HiS6 to MBP was 16 h, as judged by SDS-PAGE and Western blots against anti-His antibody. When the detagged protein was purified using subtractive IMAC, the yield was moderate at 71% although the overall recovery was high at 95%. Likewise, a yield of 79% and a recovery of 97% was obtained when digestion was performed with using "on-column" tag digestion. Oncolumn tag digestion involves cleavage of histidine tag from polyhistidine tagged proteins that are still bound to the IMAC column. Digestion of an N-terminal polyhistidine tag from HT15-MBP (1 mg/mL) by the DAPase-1 system was superiorto the results obtained with Factor Xa with a higher yield and recovery of 99% and 95%, respectively. The digestion by DAPase-1 system was faster and was complete at 5 h as opposed to 16 h for Factor Xa. The detagged MBP proteins were isolated from the digestion mixtures using a simple subtractive IMAC column procedure with the detagged protein appearing in the flowthrough and washing fractions while residual dipeptides and DAPase-1 (which was engineered to exhibit a poly-His tail) were adsorbed to the column. FPLC analysis using a MonoS cation exchanger was performed to understand and monitor the progress and time course of DAPase-1 digestion of HT15-MBP to MBP. Optimization of process variables such as temperature, protein concentration, and enzyme activity was developed for the DAPase-1 digesting system on HT15-MBP to MBP. In short, this study proved that the use of either Factor Xa or DAPase-l for the digestion of polyhistidine tags is simple and efficient and can be carried out under mild reaction conditions. Wiley Periodicals 2005-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18361/1/Removal%20of%20poly.pdf Abdullah, Norhafizah and Chase, Howard Allaker (2005) Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption. Biotechnology and Bioengineering, 92 (4). pp. 501-513. ISSN 0006-3592; ESSN: 1097-0290 http://onlinelibrary.wiley.com/wol1/doi/10.1002/bit.20633/abstract doi:10.1002/bit.20633 |
| spellingShingle | Abdullah, Norhafizah Chase, Howard Allaker Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption |
| title | Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption |
| title_full | Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption |
| title_fullStr | Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption |
| title_full_unstemmed | Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption |
| title_short | Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption |
| title_sort | removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption |
| url | http://psasir.upm.edu.my/id/eprint/18361/ http://psasir.upm.edu.my/id/eprint/18361/ http://psasir.upm.edu.my/id/eprint/18361/ http://psasir.upm.edu.my/id/eprint/18361/1/Removal%20of%20poly.pdf |