Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7
The immunodominant region of hepatitis B virus (HBV) located in the viral small surface antigen (S-HBsAg) elicits virus-neutralizing and protective antibodies. In order to develop an easy and inexpensive method to produce this region without the need for extensive purification, amino acid residues 1...
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| Format: | Article |
| Language: | English English |
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2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18325/ http://psasir.upm.edu.my/id/eprint/18325/1/Antigenicity%20and%20immunogenicity%20of%20the%20immunodominant%20region%20of%20hepatitis%20B%20surface%20antigen%20displayed%20on%20bacteriophage%20T7.pdf |
| _version_ | 1848843480409833472 |
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| author | Tan, Geok Hun Yusoff, Khatijah Seow, Heng Fong Tan, Wen Siang |
| author_facet | Tan, Geok Hun Yusoff, Khatijah Seow, Heng Fong Tan, Wen Siang |
| author_sort | Tan, Geok Hun |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The immunodominant region of hepatitis B virus (HBV) located in the viral small surface antigen (S-HBsAg) elicits virus-neutralizing and protective antibodies. In order to develop an easy and inexpensive method to produce this region without the need for extensive purification, amino acid residues 111-156 of S-HBsAg were fused to the C-terminal end of the 10B capsid protein of T7 phage. Western blotting and ELISA confirmed the expression of the recombinant protein on the surface of the phage particles. The recombinant phage exhibited the antigenic and immunogenic characteristics of HBsAg, illustrating its potential as an immunological reagent and vaccine. © 2005 Wiley-Liss, Inc. |
| first_indexed | 2025-11-15T08:15:42Z |
| format | Article |
| id | upm-18325 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:15:42Z |
| publishDate | 2005 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-183252016-01-21T02:00:39Z http://psasir.upm.edu.my/id/eprint/18325/ Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7 Tan, Geok Hun Yusoff, Khatijah Seow, Heng Fong Tan, Wen Siang The immunodominant region of hepatitis B virus (HBV) located in the viral small surface antigen (S-HBsAg) elicits virus-neutralizing and protective antibodies. In order to develop an easy and inexpensive method to produce this region without the need for extensive purification, amino acid residues 111-156 of S-HBsAg were fused to the C-terminal end of the 10B capsid protein of T7 phage. Western blotting and ELISA confirmed the expression of the recombinant protein on the surface of the phage particles. The recombinant phage exhibited the antigenic and immunogenic characteristics of HBsAg, illustrating its potential as an immunological reagent and vaccine. © 2005 Wiley-Liss, Inc. 2005-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18325/1/Antigenicity%20and%20immunogenicity%20of%20the%20immunodominant%20region%20of%20hepatitis%20B%20surface%20antigen%20displayed%20on%20bacteriophage%20T7.pdf Tan, Geok Hun and Yusoff, Khatijah and Seow, Heng Fong and Tan, Wen Siang (2005) Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7. Journal of Medical Virology, 77 (4). pp. 475-480. ISSN 0146-6615 http://dx.doi.org/10.1002/jmv.20479 Hepatitis B virus Immunology Recombinant proteins doi:10.1002/jmv.20479 English |
| spellingShingle | Hepatitis B virus Immunology Recombinant proteins Tan, Geok Hun Yusoff, Khatijah Seow, Heng Fong Tan, Wen Siang Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7 |
| title | Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7 |
| title_full | Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7 |
| title_fullStr | Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7 |
| title_full_unstemmed | Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7 |
| title_short | Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7 |
| title_sort | antigenicity and immunogenicity of the immunodominant region of hepatitis b surface antigen displayed on bacteriophage t7 |
| topic | Hepatitis B virus Immunology Recombinant proteins |
| url | http://psasir.upm.edu.my/id/eprint/18325/ http://psasir.upm.edu.my/id/eprint/18325/ http://psasir.upm.edu.my/id/eprint/18325/ http://psasir.upm.edu.my/id/eprint/18325/1/Antigenicity%20and%20immunogenicity%20of%20the%20immunodominant%20region%20of%20hepatitis%20B%20surface%20antigen%20displayed%20on%20bacteriophage%20T7.pdf |