Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells
Chemostat cultures of NS0 cell lines were carried out at dilution rates ranging from 0.8 d-1 to 0.2 d-1. Compared with the control, the viable cell density of the Bcl-2 cell line was approximately 10% higher at 0.8 d-1 and increased to 55% when the dilution rate was reduced to 0.2 d-1. As the diluti...
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| Format: | Article |
| Language: | English English |
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The Society for Biotechnology
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18322/ http://psasir.upm.edu.my/id/eprint/18322/1/Effect%20of%20Bcl.pdf |
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| author | Tey, Beng Ti Al-Rubeai, Mohamed |
| author_facet | Tey, Beng Ti Al-Rubeai, Mohamed |
| author_sort | Tey, Beng Ti |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chemostat cultures of NS0 cell lines were carried out at dilution rates ranging from 0.8 d-1 to 0.2 d-1. Compared with the control, the viable cell density of the Bcl-2 cell line was approximately 10% higher at 0.8 d-1 and increased to 55% when the dilution rate was reduced to 0.2 d-1. As the dilution rate was reduced, the viability of the two cultures diverged reaching a difference of 43% at 0.2 d-1. The specific growth rate of the control cells was the same as the dilution rate down to a value of 0.6 d-1. By contrast, the specific growth rate of Bcl-2 cells was parallel to the dilution rate down to a value as low as 0.3 d-1. For both NS0 cell lines, the G1 cell population decreased, while the S and G2/M cell populations increased as the dilution rate was reduced. The antibody titer of the control cells increased from 7 to 21 μg·ml-1 as the dilution rate was reduced from 0.8 to 0.2 d-1. With an initial increase from 2 to 15 μg·ml-1 as the dilution rate was reduced from 0.8 to 0.4 d-1, the antibody titer of the Bcl-2 cells remained constant as the dilution rate was further reduced to 0.2 d-1. A good correlation between specific antibody production rate and the percentage of G2/M cells was observed. © 2005, The Society for Biotechnology. |
| first_indexed | 2025-11-15T08:15:41Z |
| format | Article |
| id | upm-18322 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:15:41Z |
| publishDate | 2005 |
| publisher | The Society for Biotechnology |
| recordtype | eprints |
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| spelling | upm-183222016-01-21T03:45:21Z http://psasir.upm.edu.my/id/eprint/18322/ Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells Tey, Beng Ti Al-Rubeai, Mohamed Chemostat cultures of NS0 cell lines were carried out at dilution rates ranging from 0.8 d-1 to 0.2 d-1. Compared with the control, the viable cell density of the Bcl-2 cell line was approximately 10% higher at 0.8 d-1 and increased to 55% when the dilution rate was reduced to 0.2 d-1. As the dilution rate was reduced, the viability of the two cultures diverged reaching a difference of 43% at 0.2 d-1. The specific growth rate of the control cells was the same as the dilution rate down to a value of 0.6 d-1. By contrast, the specific growth rate of Bcl-2 cells was parallel to the dilution rate down to a value as low as 0.3 d-1. For both NS0 cell lines, the G1 cell population decreased, while the S and G2/M cell populations increased as the dilution rate was reduced. The antibody titer of the control cells increased from 7 to 21 μg·ml-1 as the dilution rate was reduced from 0.8 to 0.2 d-1. With an initial increase from 2 to 15 μg·ml-1 as the dilution rate was reduced from 0.8 to 0.4 d-1, the antibody titer of the Bcl-2 cells remained constant as the dilution rate was further reduced to 0.2 d-1. A good correlation between specific antibody production rate and the percentage of G2/M cells was observed. © 2005, The Society for Biotechnology. The Society for Biotechnology 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18322/1/Effect%20of%20Bcl.pdf Tey, Beng Ti and Al-Rubeai, Mohamed (2005) Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells. Journal of Bioscience and Bioengineering, 100 (3). pp. 303-310. ISSN 1389-1723 http://dx.doi.org/10.1263/jbb.100.303 Apoptosis Cell culture Animal cell biotechnology doi:10.1263/jbb.100.303 English |
| spellingShingle | Apoptosis Cell culture Animal cell biotechnology Tey, Beng Ti Al-Rubeai, Mohamed Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells |
| title | Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells |
| title_full | Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells |
| title_fullStr | Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells |
| title_full_unstemmed | Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells |
| title_short | Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells |
| title_sort | effect of bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma ns0 cells |
| topic | Apoptosis Cell culture Animal cell biotechnology |
| url | http://psasir.upm.edu.my/id/eprint/18322/ http://psasir.upm.edu.my/id/eprint/18322/ http://psasir.upm.edu.my/id/eprint/18322/ http://psasir.upm.edu.my/id/eprint/18322/1/Effect%20of%20Bcl.pdf |