Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences
Aquilaria malaccensis is a medium-sized tropical tree from the family Thymelaeaceae, and is the main 'agarwood' producer in Peninsular Malaysia. 'Agarwood' is a valuable fragrant resin, produced in the tree trunks of A. ma/accensis, often associated to pathological infections by...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18141/ http://psasir.upm.edu.my/id/eprint/18141/1/ID%2018141.pdf |
| _version_ | 1848843430410584064 |
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| author | Mohamed, Rozi Phai, Lee Jong Zali, Mohammed Syazwan Husin, Halimah |
| author_facet | Mohamed, Rozi Phai, Lee Jong Zali, Mohammed Syazwan Husin, Halimah |
| author_sort | Mohamed, Rozi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aquilaria malaccensis is a medium-sized tropical tree from the family Thymelaeaceae, and is the main 'agarwood' producer in Peninsular Malaysia. 'Agarwood' is a valuable fragrant resin, produced in the tree trunks of A. ma/accensis, often associated to pathological infections by fungi. In our attempt to understand the roles of fungi in 'agarwood' production, we isolated fungi from 'agarwood', collected from a wild A. ma/accensis population in Peninsular Malaysia. The fungal isolates were from the genera Fusarium, Trichoderma, Curvularia and Cunninghamella (order Mucorales) based on culture characteristics on agar medium. To confirm the genera, we analyzed genomic DNA from pure cultures and wood samples from which they were first isolated., ' Using universal primers in PCR, we amplified the intemal transcribed spacer
(ITS) within the ribosomal DNA (rONA) regions. Spacer sequences provided supporting evidence to the genera designation via the culture-based method. The method promised a rapid detection and identification of fungal communities directly from environmental and wood samples. |
| first_indexed | 2025-11-15T08:14:54Z |
| format | Conference or Workshop Item |
| id | upm-18141 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:14:54Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-181412013-12-10T07:37:07Z http://psasir.upm.edu.my/id/eprint/18141/ Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences Mohamed, Rozi Phai, Lee Jong Zali, Mohammed Syazwan Husin, Halimah Aquilaria malaccensis is a medium-sized tropical tree from the family Thymelaeaceae, and is the main 'agarwood' producer in Peninsular Malaysia. 'Agarwood' is a valuable fragrant resin, produced in the tree trunks of A. ma/accensis, often associated to pathological infections by fungi. In our attempt to understand the roles of fungi in 'agarwood' production, we isolated fungi from 'agarwood', collected from a wild A. ma/accensis population in Peninsular Malaysia. The fungal isolates were from the genera Fusarium, Trichoderma, Curvularia and Cunninghamella (order Mucorales) based on culture characteristics on agar medium. To confirm the genera, we analyzed genomic DNA from pure cultures and wood samples from which they were first isolated., ' Using universal primers in PCR, we amplified the intemal transcribed spacer (ITS) within the ribosomal DNA (rONA) regions. Spacer sequences provided supporting evidence to the genera designation via the culture-based method. The method promised a rapid detection and identification of fungal communities directly from environmental and wood samples. 2009 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18141/1/ID%2018141.pdf Mohamed, Rozi and Phai, Lee Jong and Zali, Mohammed Syazwan and Husin, Halimah (2009) Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences. In: International Conference on Plants, Environment and Pollution, 6 -11 July, 2009, Kayseri, Turkey. . Aquilaria malaccensis Agarwood (Wood) Recombinant DNA English |
| spellingShingle | Aquilaria malaccensis Agarwood (Wood) Recombinant DNA Mohamed, Rozi Phai, Lee Jong Zali, Mohammed Syazwan Husin, Halimah Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences |
| title | Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences |
| title_full | Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences |
| title_fullStr | Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences |
| title_full_unstemmed | Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences |
| title_short | Identification of Fungal Communities in "Agarwood' from wild Aquilaria malaccensis using rDNA Spacer Sequences |
| title_sort | identification of fungal communities in "agarwood' from wild aquilaria malaccensis using rdna spacer sequences |
| topic | Aquilaria malaccensis Agarwood (Wood) Recombinant DNA |
| url | http://psasir.upm.edu.my/id/eprint/18141/ http://psasir.upm.edu.my/id/eprint/18141/1/ID%2018141.pdf |