Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis

The aim of this study was to evaluate glycerolized bovine parietal tunica vaginalis implant in rat model. Pieces of 3_2.5 cm bovine parietal tunica vaginalis preserved in 99.5% glycerol and stored at 4 degrees C were used to repair 3_2.5 cm full thickness abdominal wall defects created in a group of...

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Main Authors: Yagoub Mohamed, Abdel Hafeez, Abu Bakar @ Zakaria, Md Zuki, Mohamad Yusof, Loqman, Yusof, Norimah, Hassan, Asnah, Mohamed Mustapha, Noordin
Format: Conference or Workshop Item
Language:English
Published: Universiti Putra Malaysia Press 2004
Online Access:http://psasir.upm.edu.my/id/eprint/17963/
http://psasir.upm.edu.my/id/eprint/17963/1/71-12.pdf
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author Yagoub Mohamed, Abdel Hafeez
Abu Bakar @ Zakaria, Md Zuki
Mohamad Yusof, Loqman
Yusof, Norimah
Hassan, Asnah
Mohamed Mustapha, Noordin
author_facet Yagoub Mohamed, Abdel Hafeez
Abu Bakar @ Zakaria, Md Zuki
Mohamad Yusof, Loqman
Yusof, Norimah
Hassan, Asnah
Mohamed Mustapha, Noordin
author_sort Yagoub Mohamed, Abdel Hafeez
building UPM Institutional Repository
collection Online Access
description The aim of this study was to evaluate glycerolized bovine parietal tunica vaginalis implant in rat model. Pieces of 3_2.5 cm bovine parietal tunica vaginalis preserved in 99.5% glycerol and stored at 4 degrees C were used to repair 3_2.5 cm full thickness abdominal wall defects created in a group of 30 male Sprague Dawley rats (300-400 g). Another group of 30 rats were underwent sham operation and used for comparison. Each group was divided into five subgroups (n=6) and sacrificed at post-surgical intervals of 1, 3, 6, 9 and 18 weeks for macroscopical, histological and mechanical evaluation. Loose adhesions were observed between the implanted graft and underlying visceral organs in 6.6% of the treated group. Histologically the graft was biocompatible and gradually replaced by the recipient fibers tissue. The graft healing tensile strength increased with time in both groups and no significant different (P>0.05) was observed between the overall means of healing tensile strength of the two groups. The outcome of this study revealed that glycerolized bovine tunica vaginalis is biocompatible surgical patch that can be used for reconstruction of soft tissue defects. However, further investigation is required regarding the glycerol preservation efficiency.
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institution Universiti Putra Malaysia
institution_category Local University
language English
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publishDate 2004
publisher Universiti Putra Malaysia Press
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spelling upm-179632018-08-20T02:05:45Z http://psasir.upm.edu.my/id/eprint/17963/ Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis Yagoub Mohamed, Abdel Hafeez Abu Bakar @ Zakaria, Md Zuki Mohamad Yusof, Loqman Yusof, Norimah Hassan, Asnah Mohamed Mustapha, Noordin The aim of this study was to evaluate glycerolized bovine parietal tunica vaginalis implant in rat model. Pieces of 3_2.5 cm bovine parietal tunica vaginalis preserved in 99.5% glycerol and stored at 4 degrees C were used to repair 3_2.5 cm full thickness abdominal wall defects created in a group of 30 male Sprague Dawley rats (300-400 g). Another group of 30 rats were underwent sham operation and used for comparison. Each group was divided into five subgroups (n=6) and sacrificed at post-surgical intervals of 1, 3, 6, 9 and 18 weeks for macroscopical, histological and mechanical evaluation. Loose adhesions were observed between the implanted graft and underlying visceral organs in 6.6% of the treated group. Histologically the graft was biocompatible and gradually replaced by the recipient fibers tissue. The graft healing tensile strength increased with time in both groups and no significant different (P>0.05) was observed between the overall means of healing tensile strength of the two groups. The outcome of this study revealed that glycerolized bovine tunica vaginalis is biocompatible surgical patch that can be used for reconstruction of soft tissue defects. However, further investigation is required regarding the glycerol preservation efficiency. Universiti Putra Malaysia Press 2004 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/17963/1/71-12.pdf Yagoub Mohamed, Abdel Hafeez and Abu Bakar @ Zakaria, Md Zuki and Mohamad Yusof, Loqman and Yusof, Norimah and Hassan, Asnah and Mohamed Mustapha, Noordin (2004) Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis. In: 11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress, 23-27 Aug. 2004, Sunway Pyramid Convention Centre, Petaling Jaya, Malaysia. (pp. 190-191).
spellingShingle Yagoub Mohamed, Abdel Hafeez
Abu Bakar @ Zakaria, Md Zuki
Mohamad Yusof, Loqman
Yusof, Norimah
Hassan, Asnah
Mohamed Mustapha, Noordin
Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis
title Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis
title_full Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis
title_fullStr Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis
title_full_unstemmed Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis
title_short Evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis
title_sort evaluation of rat soft tissue response to implantation of glycerolized bovine tunica vaginalis
url http://psasir.upm.edu.my/id/eprint/17963/
http://psasir.upm.edu.my/id/eprint/17963/1/71-12.pdf