Chromosome studies related to fertility in crossbred buffaloes in Malaysia.
Chromosome analysis was undertaken on Murrah, Swamp, F1 crossbred (Murrah x Swamp) and first generation backross (F1 female x Murrah male) buffaloes using the leukocyte culture method combined with Giemsa (G) and centromeric (C) and nucleolar organiser region (NOR) banding procedures. The F1 crossbr...
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| Format: | Conference or Workshop Item |
| Language: | English |
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1982
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17892/ http://psasir.upm.edu.my/id/eprint/17892/1/ID%2017892.pdf |
| _version_ | 1848843366672891904 |
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| author | Bongso, T. A. Hilmi, M. Jainudeen, M. R. |
| author_facet | Bongso, T. A. Hilmi, M. Jainudeen, M. R. |
| author_sort | Bongso, T. A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chromosome analysis was undertaken on Murrah, Swamp, F1 crossbred (Murrah x Swamp) and first generation backross (F1 female x Murrah male) buffaloes using the leukocyte culture method combined with Giemsa (G) and centromeric (C) and nucleolar organiser region (NOR) banding procedures. The F1 crossbred had a diploid chromosome complement(2n=49)intermediate to its two parents (Murrah,2n=50 ; Swamp, 2n=48). Two backross animals were 2n=49 and 2n=50 respectively. Construction of G,C NOR and conventional karyotypes revealed that the largest two metacentric chromosome in the Swamp buffalo resulted from a telomere-centromere tandem fusion between chromosomes 4p and 9 of the Murrah karyotype. The F1 crossbred and backross animals with 2n=49 showed a similar tandem fusion, occuring however between one member each of chromosome pairs 4p and 9. The two X chromosome (acrocentric) in female buffaloes had large masses of centromeric heterochromatin and one pair of dark bands in their arms proximal to the centromere, while in the Y chromosome of males, centromeric heterochromatin was not stained. The Y chromosome was not the smallest chromosome in the buffalo karyotype. The implications of the 2n=49 chromosome complement on fertility via inter se and backcross matings is hypothesised and discussed. |
| first_indexed | 2025-11-15T08:13:53Z |
| format | Conference or Workshop Item |
| id | upm-17892 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:13:53Z |
| publishDate | 1982 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-178922015-01-05T06:33:35Z http://psasir.upm.edu.my/id/eprint/17892/ Chromosome studies related to fertility in crossbred buffaloes in Malaysia. Bongso, T. A. Hilmi, M. Jainudeen, M. R. Chromosome analysis was undertaken on Murrah, Swamp, F1 crossbred (Murrah x Swamp) and first generation backross (F1 female x Murrah male) buffaloes using the leukocyte culture method combined with Giemsa (G) and centromeric (C) and nucleolar organiser region (NOR) banding procedures. The F1 crossbred had a diploid chromosome complement(2n=49)intermediate to its two parents (Murrah,2n=50 ; Swamp, 2n=48). Two backross animals were 2n=49 and 2n=50 respectively. Construction of G,C NOR and conventional karyotypes revealed that the largest two metacentric chromosome in the Swamp buffalo resulted from a telomere-centromere tandem fusion between chromosomes 4p and 9 of the Murrah karyotype. The F1 crossbred and backross animals with 2n=49 showed a similar tandem fusion, occuring however between one member each of chromosome pairs 4p and 9. The two X chromosome (acrocentric) in female buffaloes had large masses of centromeric heterochromatin and one pair of dark bands in their arms proximal to the centromere, while in the Y chromosome of males, centromeric heterochromatin was not stained. The Y chromosome was not the smallest chromosome in the buffalo karyotype. The implications of the 2n=49 chromosome complement on fertility via inter se and backcross matings is hypothesised and discussed. 1982 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17892/1/ID%2017892.pdf Bongso, T. A. and Hilmi, M. and Jainudeen, M. R. (1982) Chromosome studies related to fertility in crossbred buffaloes in Malaysia. In: Third Research Coordination Meeting of the Coordinated Research Programme on the 'Use of Nuclear Techniques to Improve Domestic Buffalo Production in Asia, 19-23 Apr. 1982, Serdang, Selangor. . Buffaloes - Breeding |
| spellingShingle | Buffaloes - Breeding Bongso, T. A. Hilmi, M. Jainudeen, M. R. Chromosome studies related to fertility in crossbred buffaloes in Malaysia. |
| title | Chromosome studies related to fertility in crossbred buffaloes in Malaysia. |
| title_full | Chromosome studies related to fertility in crossbred buffaloes in Malaysia. |
| title_fullStr | Chromosome studies related to fertility in crossbred buffaloes in Malaysia. |
| title_full_unstemmed | Chromosome studies related to fertility in crossbred buffaloes in Malaysia. |
| title_short | Chromosome studies related to fertility in crossbred buffaloes in Malaysia. |
| title_sort | chromosome studies related to fertility in crossbred buffaloes in malaysia. |
| topic | Buffaloes - Breeding |
| url | http://psasir.upm.edu.my/id/eprint/17892/ http://psasir.upm.edu.my/id/eprint/17892/1/ID%2017892.pdf |