Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying
Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85°C) and the heat-adaptation temperature (45 and 65 °C, 30 m...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Libertas Academica Ltd.
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17753/ http://psasir.upm.edu.my/id/eprint/17753/1/Viability%20of%20bifidobacterium%20pseudocatenulatum%20G4%20after%20spray.pdf |
| _version_ | 1848843329757773824 |
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| author | Abd Manap, Mohd Yazid Shobirin, Anis Mohamad, Rosfarizan Mustafa, Shuhaimi Wong, Stephenie Kabeir, Barka Mohammed |
| author_facet | Abd Manap, Mohd Yazid Shobirin, Anis Mohamad, Rosfarizan Mustafa, Shuhaimi Wong, Stephenie Kabeir, Barka Mohammed |
| author_sort | Abd Manap, Mohd Yazid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in
viability regardless of the air outlet temperature (75 and 85°C) and the heat-adaptation temperature (45 and 65 °C, 30 min). The use of heat-adaptation treatment to improve the thermotolerance of this strain was ineffective. On the other hand, the strain showed a superior survival at 71.65%–82.07% after freeze-drying. Viable populations of 9.319–9.487 log10 cfu/g were obtained when different
combinations of skim milk and sugar were used as cryoprotectant. However, the addition of sugars did not result in increased survival during the freeze-drying process. Hence, 10% (w/v) skim milk alone is recommended as a suitable protectant and drying medium for this strain. The residual moisture content obtained was 4.41% ± 0.44%. |
| first_indexed | 2025-11-15T08:13:18Z |
| format | Article |
| id | upm-17753 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:13:18Z |
| publishDate | 2009 |
| publisher | Libertas Academica Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-177532015-11-30T07:31:25Z http://psasir.upm.edu.my/id/eprint/17753/ Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying Abd Manap, Mohd Yazid Shobirin, Anis Mohamad, Rosfarizan Mustafa, Shuhaimi Wong, Stephenie Kabeir, Barka Mohammed Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85°C) and the heat-adaptation temperature (45 and 65 °C, 30 min). The use of heat-adaptation treatment to improve the thermotolerance of this strain was ineffective. On the other hand, the strain showed a superior survival at 71.65%–82.07% after freeze-drying. Viable populations of 9.319–9.487 log10 cfu/g were obtained when different combinations of skim milk and sugar were used as cryoprotectant. However, the addition of sugars did not result in increased survival during the freeze-drying process. Hence, 10% (w/v) skim milk alone is recommended as a suitable protectant and drying medium for this strain. The residual moisture content obtained was 4.41% ± 0.44%. Libertas Academica Ltd. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17753/1/Viability%20of%20bifidobacterium%20pseudocatenulatum%20G4%20after%20spray.pdf Abd Manap, Mohd Yazid and Shobirin, Anis and Mohamad, Rosfarizan and Mustafa, Shuhaimi and Wong, Stephenie and Kabeir, Barka Mohammed (2009) Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying. Microbiology Insights, 2 (July). pp. 1-7. ISSN 1178-6361 Skim milk Dairy products 10.4137/MBI.S2728 English |
| spellingShingle | Skim milk Dairy products Abd Manap, Mohd Yazid Shobirin, Anis Mohamad, Rosfarizan Mustafa, Shuhaimi Wong, Stephenie Kabeir, Barka Mohammed Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying |
| title | Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying |
| title_full | Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying |
| title_fullStr | Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying |
| title_full_unstemmed | Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying |
| title_short | Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying |
| title_sort | viability of bifidobacterium pseudocatenulatum g4 after spray-drying and freeze-drying |
| topic | Skim milk Dairy products |
| url | http://psasir.upm.edu.my/id/eprint/17753/ http://psasir.upm.edu.my/id/eprint/17753/ http://psasir.upm.edu.my/id/eprint/17753/1/Viability%20of%20bifidobacterium%20pseudocatenulatum%20G4%20after%20spray.pdf |