Leptospiral infections in stray dogs in Malaysia

Leptospirosis is a zoonotic disease of worldwide importance. Besides rodents, dogs have also been implicated as a source of infection to humans. Samples of urine and blood from 142 stray dogs were examined for leptospirosis by serological tests and bacterial cultures. Of these, 83% (118/142) and 33%...

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Main Authors: Abdul Mutalib, Abdul Rahim, Mohd Lila, Mohd Azmi, Bahaman, Abdul Rani, Phoumoona, T.
Format: Article
Language:English
Published: Veterinary Association Malaysia 2009
Online Access:http://psasir.upm.edu.my/id/eprint/17720/
http://psasir.upm.edu.my/id/eprint/17720/1/Leptospiral%20infection%20in%20stray%20dogs%20in%20malaysia.pdf
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author Abdul Mutalib, Abdul Rahim
Mohd Lila, Mohd Azmi
Bahaman, Abdul Rani
Phoumoona, T.
author_facet Abdul Mutalib, Abdul Rahim
Mohd Lila, Mohd Azmi
Bahaman, Abdul Rani
Phoumoona, T.
author_sort Abdul Mutalib, Abdul Rahim
building UPM Institutional Repository
collection Online Access
description Leptospirosis is a zoonotic disease of worldwide importance. Besides rodents, dogs have also been implicated as a source of infection to humans. Samples of urine and blood from 142 stray dogs were examined for leptospirosis by serological tests and bacterial cultures. Of these, 83% (118/142) and 33% (471142) of the serum samples were positive by ELISA-IgM/IgG and the microscopic agglutination test (MAT) respectively. No leptospiral isolates were obtained on culture. Leptospira interrogans serovar pomona was found to be the most predominant serovar in the dogs with an infection rate of 68% at MAT titres of ~ 100. Urine and serum samples were randomly selected for detection and identification of leptospires by the polymerase chain reaction (PCR) and low-stringency PCR assay respectively. All seropositive samples selected were positive by PCR assay. One urine sample showed the presence of leptospires based on PCR assay.
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spelling upm-177202016-01-28T07:42:44Z http://psasir.upm.edu.my/id/eprint/17720/ Leptospiral infections in stray dogs in Malaysia Abdul Mutalib, Abdul Rahim Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Phoumoona, T. Leptospirosis is a zoonotic disease of worldwide importance. Besides rodents, dogs have also been implicated as a source of infection to humans. Samples of urine and blood from 142 stray dogs were examined for leptospirosis by serological tests and bacterial cultures. Of these, 83% (118/142) and 33% (471142) of the serum samples were positive by ELISA-IgM/IgG and the microscopic agglutination test (MAT) respectively. No leptospiral isolates were obtained on culture. Leptospira interrogans serovar pomona was found to be the most predominant serovar in the dogs with an infection rate of 68% at MAT titres of ~ 100. Urine and serum samples were randomly selected for detection and identification of leptospires by the polymerase chain reaction (PCR) and low-stringency PCR assay respectively. All seropositive samples selected were positive by PCR assay. One urine sample showed the presence of leptospires based on PCR assay. Veterinary Association Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17720/1/Leptospiral%20infection%20in%20stray%20dogs%20in%20malaysia.pdf Abdul Mutalib, Abdul Rahim and Mohd Lila, Mohd Azmi and Bahaman, Abdul Rani and Phoumoona, T. (2009) Leptospiral infections in stray dogs in Malaysia. Jurnal Veterinar Malaysia, 21 (1). pp. 23-27. ISSN 9128-2506
spellingShingle Abdul Mutalib, Abdul Rahim
Mohd Lila, Mohd Azmi
Bahaman, Abdul Rani
Phoumoona, T.
Leptospiral infections in stray dogs in Malaysia
title Leptospiral infections in stray dogs in Malaysia
title_full Leptospiral infections in stray dogs in Malaysia
title_fullStr Leptospiral infections in stray dogs in Malaysia
title_full_unstemmed Leptospiral infections in stray dogs in Malaysia
title_short Leptospiral infections in stray dogs in Malaysia
title_sort leptospiral infections in stray dogs in malaysia
url http://psasir.upm.edu.my/id/eprint/17720/
http://psasir.upm.edu.my/id/eprint/17720/1/Leptospiral%20infection%20in%20stray%20dogs%20in%20malaysia.pdf