High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia.
Persistent high-risk human papillomavirus (HPV) infection is known to play an important role in the genesis of cervical cancer. Since new screening and prevention strategies, namely improved HPV testing and HPV vaccination have been aggressively promoted recently, it is crucial to investigate the HP...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Asian Pacific Organization for Cancer Prevention
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17708/ http://psasir.upm.edu.my/id/eprint/17708/1/High%20prevalence%20of%20human%20papillomavirus%20DNA%20detected%20in%20cervical%20swabs%20from%20women%20in%20Southern%20Selangor.pdf |
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| author | Chong, Pei Pei N., Asyikin M., Rusinahayati S., Halimatun Rosli, Rozita Ng, C. K. W. H., Wan Hamilton Tan, Boon Chong Nordin, Noraihan Aziz, Rohani Hassan, Faezah Abd Latiff, Latiffah Abdullah, Maha Abdul Rahman, Sabariah |
| author_facet | Chong, Pei Pei N., Asyikin M., Rusinahayati S., Halimatun Rosli, Rozita Ng, C. K. W. H., Wan Hamilton Tan, Boon Chong Nordin, Noraihan Aziz, Rohani Hassan, Faezah Abd Latiff, Latiffah Abdullah, Maha Abdul Rahman, Sabariah |
| author_sort | Chong, Pei Pei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Persistent high-risk human papillomavirus (HPV) infection is known to play an important role in the genesis of cervical cancer. Since new screening and prevention strategies, namely improved HPV testing and HPV vaccination have been aggressively promoted recently, it is crucial to investigate the HPV distribution in Malaysia in order to maximize their cost-effectiveness. This study was therefore conducted to assess the HPV type distribution in the most populous region, the state of Selangor. A total of 200 cervical swab samples were collected in two health-screening campaigns, and also from women attending obstetrics and gynecology clinics in several hospitals in Selangor. DNA extraction was performed and HPV DNA was detected via nested PCR using MY09/MY11 as outer primers and GP5+/GP6+ as inner primers which target the L1 gene of the viral genome. The purified PCR products were subjected to automated DNA sequencing to determine the HPV genotype. Out of 180 β-globin positive samples, 84 (46.7%) were positive for HPV DNA. The most common HPV type found
was high-risk oncogenic type 16 (40%), followed by HPV type 18 (3.3%), HPV 33 (1.7%), HPV 31 (0.6%), and low-risk HPV 87 (0.6%). Our study confirmed that nested PCR method is highly sensitive in detecting HPV DNA even in low risk patients. Since a relatively high prevalence rate of HPV infection was found in this population,prompt healthcare policy changes to bring about implementation of early HPV vaccination program is desirable to prevent a high incidence of cervical cancer. |
| first_indexed | 2025-11-15T08:13:06Z |
| format | Article |
| id | upm-17708 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:13:06Z |
| publishDate | 2010 |
| publisher | Asian Pacific Organization for Cancer Prevention |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-177082015-11-03T04:42:20Z http://psasir.upm.edu.my/id/eprint/17708/ High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia. Chong, Pei Pei N., Asyikin M., Rusinahayati S., Halimatun Rosli, Rozita Ng, C. K. W. H., Wan Hamilton Tan, Boon Chong Nordin, Noraihan Aziz, Rohani Hassan, Faezah Abd Latiff, Latiffah Abdullah, Maha Abdul Rahman, Sabariah Persistent high-risk human papillomavirus (HPV) infection is known to play an important role in the genesis of cervical cancer. Since new screening and prevention strategies, namely improved HPV testing and HPV vaccination have been aggressively promoted recently, it is crucial to investigate the HPV distribution in Malaysia in order to maximize their cost-effectiveness. This study was therefore conducted to assess the HPV type distribution in the most populous region, the state of Selangor. A total of 200 cervical swab samples were collected in two health-screening campaigns, and also from women attending obstetrics and gynecology clinics in several hospitals in Selangor. DNA extraction was performed and HPV DNA was detected via nested PCR using MY09/MY11 as outer primers and GP5+/GP6+ as inner primers which target the L1 gene of the viral genome. The purified PCR products were subjected to automated DNA sequencing to determine the HPV genotype. Out of 180 β-globin positive samples, 84 (46.7%) were positive for HPV DNA. The most common HPV type found was high-risk oncogenic type 16 (40%), followed by HPV type 18 (3.3%), HPV 33 (1.7%), HPV 31 (0.6%), and low-risk HPV 87 (0.6%). Our study confirmed that nested PCR method is highly sensitive in detecting HPV DNA even in low risk patients. Since a relatively high prevalence rate of HPV infection was found in this population,prompt healthcare policy changes to bring about implementation of early HPV vaccination program is desirable to prevent a high incidence of cervical cancer. Asian Pacific Organization for Cancer Prevention 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17708/1/High%20prevalence%20of%20human%20papillomavirus%20DNA%20detected%20in%20cervical%20swabs%20from%20women%20in%20Southern%20Selangor.pdf Chong, Pei Pei and N., Asyikin and M., Rusinahayati and S., Halimatun and Rosli, Rozita and Ng, C. K. and W. H., Wan Hamilton and Tan, Boon Chong and Nordin, Noraihan and Aziz, Rohani and Hassan, Faezah and Abd Latiff, Latiffah and Abdullah, Maha and Abdul Rahman, Sabariah (2010) High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia. Asian Pacific Journal of Cancer Prevention, 11 (6). pp. 1645-1651. ISSN 1513-7368 English |
| spellingShingle | Chong, Pei Pei N., Asyikin M., Rusinahayati S., Halimatun Rosli, Rozita Ng, C. K. W. H., Wan Hamilton Tan, Boon Chong Nordin, Noraihan Aziz, Rohani Hassan, Faezah Abd Latiff, Latiffah Abdullah, Maha Abdul Rahman, Sabariah High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia. |
| title | High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia. |
| title_full | High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia. |
| title_fullStr | High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia. |
| title_full_unstemmed | High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia. |
| title_short | High prevalence of human papillomavirus DNA detected in cervical swabs from women in Southern Selangor, Malaysia. |
| title_sort | high prevalence of human papillomavirus dna detected in cervical swabs from women in southern selangor, malaysia. |
| url | http://psasir.upm.edu.my/id/eprint/17708/ http://psasir.upm.edu.my/id/eprint/17708/1/High%20prevalence%20of%20human%20papillomavirus%20DNA%20detected%20in%20cervical%20swabs%20from%20women%20in%20Southern%20Selangor.pdf |