Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch
Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Bentham Science Publishers
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17701/ |
| _version_ | 1848843315093438464 |
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| author | Siew, Ling Hii Tau, Chuan Ling Mohamad, Rosfarizan Ariff, Arbakariya |
| author_facet | Siew, Ling Hii Tau, Chuan Ling Mohamad, Rosfarizan Ariff, Arbakariya |
| author_sort | Siew, Ling Hii |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation
was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to
search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of carbon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified mineral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as compared to ‘Ohba-Ueda’ medium. This bacterium was found superior in pullulanase production using sago starch and peptone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30oC. |
| first_indexed | 2025-11-15T08:13:04Z |
| format | Article |
| id | upm-17701 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:13:04Z |
| publishDate | 2008 |
| publisher | Bentham Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-177012011-12-01T05:21:41Z http://psasir.upm.edu.my/id/eprint/17701/ Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch Siew, Ling Hii Tau, Chuan Ling Mohamad, Rosfarizan Ariff, Arbakariya Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of carbon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified mineral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as compared to ‘Ohba-Ueda’ medium. This bacterium was found superior in pullulanase production using sago starch and peptone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30oC. Bentham Science Publishers 2008 Article PeerReviewed Siew, Ling Hii and Tau, Chuan Ling and Mohamad, Rosfarizan and Ariff, Arbakariya (2008) Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch. The Open Biotechnology Journal, 3. pp. 1-8. ISSN 1874-0707 Pullulanase Fermentation English |
| spellingShingle | Pullulanase Fermentation Siew, Ling Hii Tau, Chuan Ling Mohamad, Rosfarizan Ariff, Arbakariya Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch |
| title | Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch |
| title_full | Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch |
| title_fullStr | Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch |
| title_full_unstemmed | Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch |
| title_short | Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch |
| title_sort | enhancement of extracellular pullulanase production by raoultella planticola dsmz 4617 using optimized medium based on sago starch |
| topic | Pullulanase Fermentation |
| url | http://psasir.upm.edu.my/id/eprint/17701/ |