Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada)
Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nuc...
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| Format: | Thesis |
| Language: | English |
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2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/177/ http://psasir.upm.edu.my/id/eprint/177/1/549022_FBSB_2006_11.pdf |
| _version_ | 1848838704684072960 |
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| author | Mohd Mohidin, Taznim Begam |
| author_facet | Mohd Mohidin, Taznim Begam |
| author_sort | Mohd Mohidin, Taznim Begam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family
Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other
known paramyxoviruses. In this study, the region(s) involved in the interaction between
this exceptionally large P protein with its nucleocapsid (N) protein was investigated in
vivo using the yeast two-hybrid system. Deletion analysis was used to map the domain(s)
of both the N and P proteins involved in N-P and P-N interactions. Mapping of the
domains of N protein involved in its interaction with the P protein revealed that the Cterminal
30 amino acids (423-452 residues) are crucial for N-P interaction. However,
mapping of the domains of P protein involved in the P-N association demonstrated that
both the C-terminal 63 amino acids (470-532 residues) and the immediate N-terminal 62
amino acids (1-62 residues) simultaneously play a major role. Comparison of these
findings with other studies indicates that paramyxoviruses are different in terms of interaction domains(s) between these two essential viral proteins involved in genome
replication. |
| first_indexed | 2025-11-15T06:59:47Z |
| format | Thesis |
| id | upm-177 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:59:47Z |
| publishDate | 2006 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1772021-08-18T08:25:50Z http://psasir.upm.edu.my/id/eprint/177/ Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) Mohd Mohidin, Taznim Begam Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nucleocapsid (N) protein was investigated in vivo using the yeast two-hybrid system. Deletion analysis was used to map the domain(s) of both the N and P proteins involved in N-P and P-N interactions. Mapping of the domains of N protein involved in its interaction with the P protein revealed that the Cterminal 30 amino acids (423-452 residues) are crucial for N-P interaction. However, mapping of the domains of P protein involved in the P-N association demonstrated that both the C-terminal 63 amino acids (470-532 residues) and the immediate N-terminal 62 amino acids (1-62 residues) simultaneously play a major role. Comparison of these findings with other studies indicates that paramyxoviruses are different in terms of interaction domains(s) between these two essential viral proteins involved in genome replication. 2006-02 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/177/1/549022_FBSB_2006_11.pdf Mohd Mohidin, Taznim Begam (2006) Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada). Masters thesis, Universiti Putra Malaysia. Nipah virus - Research |
| spellingShingle | Nipah virus - Research Mohd Mohidin, Taznim Begam Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title | Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_full | Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_fullStr | Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_full_unstemmed | Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_short | Specific interactions betweeen Nipah virus nucleocapsid (N) protein and Phospho-(P) protein using the yeast two-hybrid system (vector page tiada) |
| title_sort | specific interactions betweeen nipah virus nucleocapsid (n) protein and phospho-(p) protein using the yeast two-hybrid system (vector page tiada) |
| topic | Nipah virus - Research |
| url | http://psasir.upm.edu.my/id/eprint/177/ http://psasir.upm.edu.my/id/eprint/177/1/549022_FBSB_2006_11.pdf |