Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR
The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetab...
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| Format: | Article |
| Language: | English |
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Springer
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17600/ http://psasir.upm.edu.my/id/eprint/17600/1/Virulotyping%20of%20Salmonella%20enterica%20subsp.pdf |
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| author | Khoo, Chai Hoon Cheah, Yoke Kqueen Lee, Learn Han Sim, Jiun Horng Awang Salleh, Noorzaleha Mohd Sidik, Shiran Radu, Son Sukardi, Sabrina |
| author_facet | Khoo, Chai Hoon Cheah, Yoke Kqueen Lee, Learn Han Sim, Jiun Horng Awang Salleh, Noorzaleha Mohd Sidik, Shiran Radu, Son Sukardi, Sabrina |
| author_sort | Khoo, Chai Hoon |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and specific multiplex PCR assay. The assay encompasses a total of 19 Salmonella pathogenicity islands genes and a quorum sensing gene (sdiA) in three multiplex reaction sets. A total of 114 Salmonella enterica isolates belonging to 38 different serovars were tested. Each isolate in under this study was found to possess up to 70% of the virulence genes tested and exhibited variable pathogenicity gene patterns. Reproducibility of the multiplex PCR assay was found to be 100% and the detection limit of the optimized multiplex PCR was tested with lowest detectable concentration of DNA 0.8 pg μl−1. This study demonstrated various Salmonella pathogenicity island virulence gene patterns even within the same serovar. This sets of multiplex PCR system provide a fast and reliable typing approach based on Salmonella pathogenicity islands, thus enabling an effective monitoring of emerging pathogenic Salmonella strains as an additional tool in Salmonella surveillance studies. |
| first_indexed | 2025-11-15T08:12:37Z |
| format | Article |
| id | upm-17600 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:12:37Z |
| publishDate | 2009 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-176002016-10-13T07:26:34Z http://psasir.upm.edu.my/id/eprint/17600/ Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR Khoo, Chai Hoon Cheah, Yoke Kqueen Lee, Learn Han Sim, Jiun Horng Awang Salleh, Noorzaleha Mohd Sidik, Shiran Radu, Son Sukardi, Sabrina The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and specific multiplex PCR assay. The assay encompasses a total of 19 Salmonella pathogenicity islands genes and a quorum sensing gene (sdiA) in three multiplex reaction sets. A total of 114 Salmonella enterica isolates belonging to 38 different serovars were tested. Each isolate in under this study was found to possess up to 70% of the virulence genes tested and exhibited variable pathogenicity gene patterns. Reproducibility of the multiplex PCR assay was found to be 100% and the detection limit of the optimized multiplex PCR was tested with lowest detectable concentration of DNA 0.8 pg μl−1. This study demonstrated various Salmonella pathogenicity island virulence gene patterns even within the same serovar. This sets of multiplex PCR system provide a fast and reliable typing approach based on Salmonella pathogenicity islands, thus enabling an effective monitoring of emerging pathogenic Salmonella strains as an additional tool in Salmonella surveillance studies. Springer 2009-06-30 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17600/1/Virulotyping%20of%20Salmonella%20enterica%20subsp.pdf Khoo, Chai Hoon and Cheah, Yoke Kqueen and Lee, Learn Han and Sim, Jiun Horng and Awang Salleh, Noorzaleha and Mohd Sidik, Shiran and Radu, Son and Sukardi, Sabrina (2009) Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR. Antonie van Leeuwenhoek, 96 (4). pp. 441-457. ISSN 0003-6072; ESSN: 1572-9699 10.1007/s10482-009-9358-z |
| spellingShingle | Khoo, Chai Hoon Cheah, Yoke Kqueen Lee, Learn Han Sim, Jiun Horng Awang Salleh, Noorzaleha Mohd Sidik, Shiran Radu, Son Sukardi, Sabrina Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR |
| title | Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR |
| title_full | Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR |
| title_fullStr | Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR |
| title_full_unstemmed | Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR |
| title_short | Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR |
| title_sort | virulotyping of salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in malaysia using multiplex-pcr |
| url | http://psasir.upm.edu.my/id/eprint/17600/ http://psasir.upm.edu.my/id/eprint/17600/ http://psasir.upm.edu.my/id/eprint/17600/1/Virulotyping%20of%20Salmonella%20enterica%20subsp.pdf |