The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli
The hypervariable region (HVR) of VP2 protein of infectious bursal disease virus (IBDV) elicits neutralising antibodies, but it is highly hydrophobic and tends to form inclusion bodies when expressed in Escherichia coli. To improve its solubility, the VP2(HVR) was fused to the C-terminal end of Newc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17251/ |
| _version_ | 1848843190096887808 |
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| author | Saadun, Rafidah Wen, Siang Tan Omar, Abdul Rahman Bejo, Mohd. Hair Eshaghi, Majid Yusoff, Khatijah |
| author_facet | Saadun, Rafidah Wen, Siang Tan Omar, Abdul Rahman Bejo, Mohd. Hair Eshaghi, Majid Yusoff, Khatijah |
| author_sort | Saadun, Rafidah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The hypervariable region (HVR) of VP2 protein of infectious bursal disease virus (IBDV) elicits neutralising antibodies, but it is highly hydrophobic and tends to form inclusion bodies when expressed in Escherichia coli. To improve its solubility, the VP2(HVR) was fused to the C-terminal end of Newcastle disease virus (NDV) nucleocapsid (NP) protein and expressed in E. coli TOP 10 cells under the control of trc promoter. However, the fusion protein, NP-VP2(HVR)-trc, aggregated into insoluble inclusion bodies in the host cells. Therefore the coding region of NP-VP2(HVR) was sub-cloned into expression vectors containing the T7 promoter. The solubility of the NP-VP2(HVR)-T7 fusion proteins improved dramatically in E. coli BL21 (DE3), BL21 (SI) and Origami B cells. |
| first_indexed | 2025-11-15T08:11:05Z |
| format | Article |
| id | upm-17251 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:11:05Z |
| publishDate | 2008 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-172512015-09-18T03:01:04Z http://psasir.upm.edu.my/id/eprint/17251/ The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli Saadun, Rafidah Wen, Siang Tan Omar, Abdul Rahman Bejo, Mohd. Hair Eshaghi, Majid Yusoff, Khatijah The hypervariable region (HVR) of VP2 protein of infectious bursal disease virus (IBDV) elicits neutralising antibodies, but it is highly hydrophobic and tends to form inclusion bodies when expressed in Escherichia coli. To improve its solubility, the VP2(HVR) was fused to the C-terminal end of Newcastle disease virus (NDV) nucleocapsid (NP) protein and expressed in E. coli TOP 10 cells under the control of trc promoter. However, the fusion protein, NP-VP2(HVR)-trc, aggregated into insoluble inclusion bodies in the host cells. Therefore the coding region of NP-VP2(HVR) was sub-cloned into expression vectors containing the T7 promoter. The solubility of the NP-VP2(HVR)-T7 fusion proteins improved dramatically in E. coli BL21 (DE3), BL21 (SI) and Origami B cells. Universiti Putra Malaysia Press 2008-02 Article PeerReviewed Saadun, Rafidah and Wen, Siang Tan and Omar, Abdul Rahman and Bejo, Mohd. Hair and Eshaghi, Majid and Yusoff, Khatijah (2008) The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli. Pertanika Journal of Tropical Agricultural Science, 31 (1). pp. 91-99. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2031%20(1)%20Feb.%202008/13%20page%2091-99.pdf English |
| spellingShingle | Saadun, Rafidah Wen, Siang Tan Omar, Abdul Rahman Bejo, Mohd. Hair Eshaghi, Majid Yusoff, Khatijah The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli |
| title | The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli |
| title_full | The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli |
| title_fullStr | The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli |
| title_full_unstemmed | The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli |
| title_short | The nucleocapsid protein of Newcastle disease virus promotes solubility of the VP2 hypervariable region of infectious bursal disease virus in Escherichia coli |
| title_sort | nucleocapsid protein of newcastle disease virus promotes solubility of the vp2 hypervariable region of infectious bursal disease virus in escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/17251/ http://psasir.upm.edu.my/id/eprint/17251/ |