The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts
The cytotoxic effect of crude extracts of three Ganoderma species (G. lucidum, G. tropicum, and G. tsugae) was screened against the human estrogen-independent cell line, MDA-MB-435 using a microculture tetrazolium (MTT) assay. The crude extract obtained from the fruiting body of Ganoderma lucidum by...
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| Format: | Article |
| Language: | English |
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Begell House
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17248/ http://psasir.upm.edu.my/id/eprint/17248/1/The%20nature%20of%20apoptosis%20of%20human%20breast%20cancer%20cells%20induced%20by%20three%20species%20of%20genus%20Ganoderma%20P.%20Karst.%20%28Aphyllophoromycetideae%29%20crude%20extracts.pdf |
| _version_ | 1848843189221326848 |
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| author | Choong, Yew Keong Mohamed Mustapha, Noordin Mohamed, Suhaila Ali, Abdul Manaf Umar, Nor Aini Tong, Chow Chin |
| author_facet | Choong, Yew Keong Mohamed Mustapha, Noordin Mohamed, Suhaila Ali, Abdul Manaf Umar, Nor Aini Tong, Chow Chin |
| author_sort | Choong, Yew Keong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The cytotoxic effect of crude extracts of three Ganoderma species (G. lucidum, G. tropicum, and G. tsugae) was screened against the human estrogen-independent cell line, MDA-MB-435 using a microculture tetrazolium (MTT) assay. The crude extract obtained from the fruiting body of Ganoderma lucidum by hot water extraction procedures had IC50 values ranging from 140 to 350 μg mL−1. Acridine-orange/propidium iodine staining, electron microscopy, and transmission electron microscopy showed that the crude extract caused both apoptosis and necrosis. The G. lucidum mycelial crude extract was most effective, with an IC50 value of 148 μg mL−1. |
| first_indexed | 2025-11-15T08:11:04Z |
| format | Article |
| id | upm-17248 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:11:04Z |
| publishDate | 2008 |
| publisher | Begell House |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-172482016-04-08T07:34:48Z http://psasir.upm.edu.my/id/eprint/17248/ The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts Choong, Yew Keong Mohamed Mustapha, Noordin Mohamed, Suhaila Ali, Abdul Manaf Umar, Nor Aini Tong, Chow Chin The cytotoxic effect of crude extracts of three Ganoderma species (G. lucidum, G. tropicum, and G. tsugae) was screened against the human estrogen-independent cell line, MDA-MB-435 using a microculture tetrazolium (MTT) assay. The crude extract obtained from the fruiting body of Ganoderma lucidum by hot water extraction procedures had IC50 values ranging from 140 to 350 μg mL−1. Acridine-orange/propidium iodine staining, electron microscopy, and transmission electron microscopy showed that the crude extract caused both apoptosis and necrosis. The G. lucidum mycelial crude extract was most effective, with an IC50 value of 148 μg mL−1. Begell House 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17248/1/The%20nature%20of%20apoptosis%20of%20human%20breast%20cancer%20cells%20induced%20by%20three%20species%20of%20genus%20Ganoderma%20P.%20Karst.%20%28Aphyllophoromycetideae%29%20crude%20extracts.pdf Choong, Yew Keong and Mohamed Mustapha, Noordin and Mohamed, Suhaila and Ali, Abdul Manaf and Umar, Nor Aini and Tong, Chow Chin (2008) The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts. International Journal of Medicinal Mushrooms, 10 (2). pp. 115-125. ISSN 1521-9437; ESSN: 1940-4344 http://www.dl.begellhouse.com/journals/708ae68d64b17c52,26c6b3643d4e6577,60240982371c6427.html 10.1615/IntJMedMushr.v10.i2.20 |
| spellingShingle | Choong, Yew Keong Mohamed Mustapha, Noordin Mohamed, Suhaila Ali, Abdul Manaf Umar, Nor Aini Tong, Chow Chin The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts |
| title | The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts |
| title_full | The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts |
| title_fullStr | The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts |
| title_full_unstemmed | The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts |
| title_short | The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts |
| title_sort | nature of apoptosis of human breast cancer cells induced by three species of genus ganoderma p. karst. (aphyllophoromycetideae) crude extracts |
| url | http://psasir.upm.edu.my/id/eprint/17248/ http://psasir.upm.edu.my/id/eprint/17248/ http://psasir.upm.edu.my/id/eprint/17248/ http://psasir.upm.edu.my/id/eprint/17248/1/The%20nature%20of%20apoptosis%20of%20human%20breast%20cancer%20cells%20induced%20by%20three%20species%20of%20genus%20Ganoderma%20P.%20Karst.%20%28Aphyllophoromycetideae%29%20crude%20extracts.pdf |