The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen
This study was conducted to investigate and to compare liver perturbation following administration of low doses of diclofenac and ibuprofen to rats. Hundred and forty-four male Sprague Dawley rats were dosed with 3, 5 and 10 mg kg-1 diclofenac and ibuprofen in saline via intraperitoneal injection fo...
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Asian Network for Scientific Information
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17179/ http://psasir.upm.edu.my/id/eprint/17179/1/The%20histomorphological%20analysis%20of%20liver%20following%20administration%20of%20low%20doses%20of%20diclofenac%20and%20ibuprofen.pdf |
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| author | Mohanambal, Moorthy Fakurazi, Sharida Ithnin, Hairuszah |
| author_facet | Mohanambal, Moorthy Fakurazi, Sharida Ithnin, Hairuszah |
| author_sort | Mohanambal, Moorthy |
| building | UPM Institutional Repository |
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| description | This study was conducted to investigate and to compare liver perturbation following administration of low doses of diclofenac and ibuprofen to rats. Hundred and forty-four male Sprague Dawley rats were dosed with 3, 5 and 10 mg kg-1 diclofenac and ibuprofen in saline via intraperitoneal injection for 15 days. The control group was administered with saline in a similar manner. Four rats were euthanised every three days until day 15. Livers were removed, cleaned and a section across the right lobe was taken and fixed in 10% formalin for light microscopy and TUNEL assay. One-way ANOVA was used to analyse the data. p<0.05 was accepted as significant in this study. Three, 5 and 10 mg kg-1 diclofenac-treated groups and 5, 10 mg kg-1 ibuprofen administered groups showed significant changes compared to saline-treated group at day 15. The changes include presence of focal infiltration by neutrophils and lymphocytes and mild focal necrosis. In 5 and 10 mg kg-1 diclofenac administered groups and 10 mg kg-1 ibuprofen-treated group, apoptotic cells were seen around the perivenular regions (PV) only at day 15. However, not all the PVs were present with apoptotic cells. This study has shown that, diclofenac is probably more potent in inducing histomorphological changes at low doses. Both the drugs seem to exert time and dose dependent liver morphological alterations to the treated animals.
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| first_indexed | 2025-11-15T08:10:46Z |
| format | Article |
| id | upm-17179 |
| institution | Universiti Putra Malaysia |
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| language | English English |
| last_indexed | 2025-11-15T08:10:46Z |
| publishDate | 2009 |
| publisher | Asian Network for Scientific Information |
| recordtype | eprints |
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| spelling | upm-171792015-10-23T02:32:28Z http://psasir.upm.edu.my/id/eprint/17179/ The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen Mohanambal, Moorthy Fakurazi, Sharida Ithnin, Hairuszah This study was conducted to investigate and to compare liver perturbation following administration of low doses of diclofenac and ibuprofen to rats. Hundred and forty-four male Sprague Dawley rats were dosed with 3, 5 and 10 mg kg-1 diclofenac and ibuprofen in saline via intraperitoneal injection for 15 days. The control group was administered with saline in a similar manner. Four rats were euthanised every three days until day 15. Livers were removed, cleaned and a section across the right lobe was taken and fixed in 10% formalin for light microscopy and TUNEL assay. One-way ANOVA was used to analyse the data. p<0.05 was accepted as significant in this study. Three, 5 and 10 mg kg-1 diclofenac-treated groups and 5, 10 mg kg-1 ibuprofen administered groups showed significant changes compared to saline-treated group at day 15. The changes include presence of focal infiltration by neutrophils and lymphocytes and mild focal necrosis. In 5 and 10 mg kg-1 diclofenac administered groups and 10 mg kg-1 ibuprofen-treated group, apoptotic cells were seen around the perivenular regions (PV) only at day 15. However, not all the PVs were present with apoptotic cells. This study has shown that, diclofenac is probably more potent in inducing histomorphological changes at low doses. Both the drugs seem to exert time and dose dependent liver morphological alterations to the treated animals. Asian Network for Scientific Information 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17179/1/The%20histomorphological%20analysis%20of%20liver%20following%20administration%20of%20low%20doses%20of%20diclofenac%20and%20ibuprofen.pdf Mohanambal, Moorthy and Fakurazi, Sharida and Ithnin, Hairuszah (2009) The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen. Journal of Biological Sciences, 9 (7). pp. 676-681. ISSN 1727-3048, ESSN: 1812-5719 http://scialert.net/jindex.php?issn=1727-3048 Liver - Research Apoptosis - Liver - Animal Diclofenac - Low doses 10.3923/jbs.2009.676.681 English |
| spellingShingle | Liver - Research Apoptosis - Liver - Animal Diclofenac - Low doses Mohanambal, Moorthy Fakurazi, Sharida Ithnin, Hairuszah The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen |
| title | The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen |
| title_full | The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen |
| title_fullStr | The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen |
| title_full_unstemmed | The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen |
| title_short | The histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen |
| title_sort | histomorphological analysis of liver following administration of low doses of diclofenac and ibuprofen |
| topic | Liver - Research Apoptosis - Liver - Animal Diclofenac - Low doses |
| url | http://psasir.upm.edu.my/id/eprint/17179/ http://psasir.upm.edu.my/id/eprint/17179/ http://psasir.upm.edu.my/id/eprint/17179/ http://psasir.upm.edu.my/id/eprint/17179/1/The%20histomorphological%20analysis%20of%20liver%20following%20administration%20of%20low%20doses%20of%20diclofenac%20and%20ibuprofen.pdf |