In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell
Background: Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear. Results: Strobilanthes cripus was able to reduce cell viability and proliferation in M...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17144/ http://psasir.upm.edu.my/id/eprint/17144/1/In%20vitro%20cytotoxicity%20of%20Strobilanthes%20crispus%20ethanol%20extract%20on%20hormone%20dependent%20human%20breast%20adenocarcinoma%20MCF-7%20cell.pdf |
| _version_ | 1848843160625610752 |
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| author | Chong, Hueh Zan Rahmat, Asmah Yeap, Swee Keong Md. Akim, Abdah Mohamed Alitheen, Noorjahan Banu Othman, Fauziah Ee, Gwendoline Cheng Lian |
| author_facet | Chong, Hueh Zan Rahmat, Asmah Yeap, Swee Keong Md. Akim, Abdah Mohamed Alitheen, Noorjahan Banu Othman, Fauziah Ee, Gwendoline Cheng Lian |
| author_sort | Chong, Hueh Zan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear. Results: Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC50 of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study. Conclusion: Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway. |
| first_indexed | 2025-11-15T08:10:37Z |
| format | Article |
| id | upm-17144 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:10:37Z |
| publishDate | 2012 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-171442016-04-08T03:49:28Z http://psasir.upm.edu.my/id/eprint/17144/ In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell Chong, Hueh Zan Rahmat, Asmah Yeap, Swee Keong Md. Akim, Abdah Mohamed Alitheen, Noorjahan Banu Othman, Fauziah Ee, Gwendoline Cheng Lian Background: Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear. Results: Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC50 of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study. Conclusion: Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway. BioMed Central 2012-04-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17144/1/In%20vitro%20cytotoxicity%20of%20Strobilanthes%20crispus%20ethanol%20extract%20on%20hormone%20dependent%20human%20breast%20adenocarcinoma%20MCF-7%20cell.pdf Chong, Hueh Zan and Rahmat, Asmah and Yeap, Swee Keong and Md. Akim, Abdah and Mohamed Alitheen, Noorjahan Banu and Othman, Fauziah and Ee, Gwendoline Cheng Lian (2012) In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell. BMC Complementary and Alternative Medicine, 12. art. no. 35. pp. 1-10. ISSN 1472-6882 10.1186/1472-6882-12-35 |
| spellingShingle | Chong, Hueh Zan Rahmat, Asmah Yeap, Swee Keong Md. Akim, Abdah Mohamed Alitheen, Noorjahan Banu Othman, Fauziah Ee, Gwendoline Cheng Lian In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
| title | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
| title_full | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
| title_fullStr | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
| title_full_unstemmed | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
| title_short | In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell |
| title_sort | in vitro cytotoxicity of strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma mcf-7 cell |
| url | http://psasir.upm.edu.my/id/eprint/17144/ http://psasir.upm.edu.my/id/eprint/17144/ http://psasir.upm.edu.my/id/eprint/17144/1/In%20vitro%20cytotoxicity%20of%20Strobilanthes%20crispus%20ethanol%20extract%20on%20hormone%20dependent%20human%20breast%20adenocarcinoma%20MCF-7%20cell.pdf |