The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes
The effect of Morinda citrifolia fruit (MCFE), Momordica charantia (MCE) and Centella asiatica (CAE) extracts on lipoprotein lipase (LPL) activity, proliferation and differentiation of 3T3-L1 preadipocytes was investigated. The inhibitory effect of the extracts on LPL activity was determined in vitr...
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| Format: | Article |
| Language: | English |
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Wiley-Blackwell
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17139/ |
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| author | Sahib, Najla Gooda Abdul Hamid, Azizah Kitts, David Purnama, Monica Saari, Nazamid Abas, Faridah |
| author_facet | Sahib, Najla Gooda Abdul Hamid, Azizah Kitts, David Purnama, Monica Saari, Nazamid Abas, Faridah |
| author_sort | Sahib, Najla Gooda |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The effect of Morinda citrifolia fruit (MCFE), Momordica charantia (MCE) and Centella asiatica (CAE) extracts on lipoprotein lipase (LPL) activity, proliferation and differentiation of 3T3-L1 preadipocytes was investigated. The inhibitory effect of the extracts on LPL activity was determined in vitro. The extent of cell proliferation was determined utilizing 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and the degree of differentiation was measured by Oil Red O staining assay. Extracts were analyzed for total phenolic compounds and flavonoids utilizing Folin–Ciocalteu assay and high-performance liquid chromatography, respectively. Results showed that, at 1 mg/mL, MCFE demonstrated the highest inhibitory effect (21.4 ± 2.3%), compared to the other extracts tested. On the other hand, MCE demonstrated the highest inhibitory effect on both proliferation and differentiation of preadipocytes (LC50of 1.6 mg/L). LC50 refers to the concentration of extracts (mg/mL) to cause a 50% reduction in viable cells. Results also revealed that all extracts consisted of high concentration of phenolic compounds, with catechin and epicatechin predominantly present in all three extracts, that maybe responsible for the activities measured. |
| first_indexed | 2025-11-15T08:10:35Z |
| format | Article |
| id | upm-17139 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:10:35Z |
| publishDate | 2010 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
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| spelling | upm-171392012-11-01T04:00:55Z http://psasir.upm.edu.my/id/eprint/17139/ The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes Sahib, Najla Gooda Abdul Hamid, Azizah Kitts, David Purnama, Monica Saari, Nazamid Abas, Faridah The effect of Morinda citrifolia fruit (MCFE), Momordica charantia (MCE) and Centella asiatica (CAE) extracts on lipoprotein lipase (LPL) activity, proliferation and differentiation of 3T3-L1 preadipocytes was investigated. The inhibitory effect of the extracts on LPL activity was determined in vitro. The extent of cell proliferation was determined utilizing 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and the degree of differentiation was measured by Oil Red O staining assay. Extracts were analyzed for total phenolic compounds and flavonoids utilizing Folin–Ciocalteu assay and high-performance liquid chromatography, respectively. Results showed that, at 1 mg/mL, MCFE demonstrated the highest inhibitory effect (21.4 ± 2.3%), compared to the other extracts tested. On the other hand, MCE demonstrated the highest inhibitory effect on both proliferation and differentiation of preadipocytes (LC50of 1.6 mg/L). LC50 refers to the concentration of extracts (mg/mL) to cause a 50% reduction in viable cells. Results also revealed that all extracts consisted of high concentration of phenolic compounds, with catechin and epicatechin predominantly present in all three extracts, that maybe responsible for the activities measured. Wiley-Blackwell 2010 Article PeerReviewed Sahib, Najla Gooda and Abdul Hamid, Azizah and Kitts, David and Purnama, Monica and Saari, Nazamid and Abas, Faridah (2010) The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes. Journal of Food Biochemistry, 34 (4). pp. 1186-1205. ISSN 0145-8884 Medicinal plants Botany, Medical 10.1111/j.1745-4514.2010.00444.x English |
| spellingShingle | Medicinal plants Botany, Medical Sahib, Najla Gooda Abdul Hamid, Azizah Kitts, David Purnama, Monica Saari, Nazamid Abas, Faridah The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes |
| title | The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes |
| title_full | The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes |
| title_fullStr | The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes |
| title_full_unstemmed | The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes |
| title_short | The effects of Morinda Citrifolia, Momordica Charantia and Centella Asiatica Extracts on Lipoprotein Lipase and 3T3-L1 Preadipocytes |
| title_sort | effects of morinda citrifolia, momordica charantia and centella asiatica extracts on lipoprotein lipase and 3t3-l1 preadipocytes |
| topic | Medicinal plants Botany, Medical |
| url | http://psasir.upm.edu.my/id/eprint/17139/ http://psasir.upm.edu.my/id/eprint/17139/ |