The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling
HBcAg (hepatitis B core antigen) is a nanoplex bioproduct that has a great potential in the development of therapeutic drugs and vaccines. In the present study, a continuous-flow bead milling for the disruption of Escherichia coli was optimized and a direct recovery protocol to isolate the recombina...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Portland Press
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17060/ http://psasir.upm.edu.my/id/eprint/17060/1/The%20direct%20recovery%20of%20recombinant%20hepatitis%20B%20core%20antigen%20from%20disruptate%20derived%20from%20continuous.pdf |
| _version_ | 1848843136057475072 |
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| author | Ho, Chin Woi Tan, Wen Siang Kamarudin, Suryani Ling, Tau Chuan Tey, Beng Ti |
| author_facet | Ho, Chin Woi Tan, Wen Siang Kamarudin, Suryani Ling, Tau Chuan Tey, Beng Ti |
| author_sort | Ho, Chin Woi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | HBcAg (hepatitis B core antigen) is a nanoplex bioproduct that has a great potential in the development of therapeutic drugs and vaccines. In the present study, a continuous-flow bead milling for the disruption of Escherichia coli was optimized and a direct recovery protocol to isolate the recombinant HBcAg from the unclarified E. coli disruptate was developed. The optimal condition for continuous-flow bead milling for the release of HBcAg from E. coli was achieved at a feed flow rate of 15 litres/h, biomass concentration of 10% [ww/v (wet weight/vol.)] and impeller tip speed of 14 m/s. The sucrose-density-gradient analysis showed that the particulate form of the HBcAg released by this optimal condition is still preserved. In the direct purification of HBcAg from the unclarified disruptate, the AE-EBAC (anion-exchange expanded-bed adsorption chromatography) technique was employed. A 54% adsorption and 50.7% recovery of HBcAg were achieved in this direct recovery process. The purity of HBcAg recovered was 49.8%, which corresponds to a purification factor of 2.0. ELISA showed that the HBcAg recovered is functionally active. |
| first_indexed | 2025-11-15T08:10:13Z |
| format | Article |
| id | upm-17060 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:10:13Z |
| publishDate | 2008 |
| publisher | Portland Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-170602016-11-30T09:36:01Z http://psasir.upm.edu.my/id/eprint/17060/ The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling Ho, Chin Woi Tan, Wen Siang Kamarudin, Suryani Ling, Tau Chuan Tey, Beng Ti HBcAg (hepatitis B core antigen) is a nanoplex bioproduct that has a great potential in the development of therapeutic drugs and vaccines. In the present study, a continuous-flow bead milling for the disruption of Escherichia coli was optimized and a direct recovery protocol to isolate the recombinant HBcAg from the unclarified E. coli disruptate was developed. The optimal condition for continuous-flow bead milling for the release of HBcAg from E. coli was achieved at a feed flow rate of 15 litres/h, biomass concentration of 10% [ww/v (wet weight/vol.)] and impeller tip speed of 14 m/s. The sucrose-density-gradient analysis showed that the particulate form of the HBcAg released by this optimal condition is still preserved. In the direct purification of HBcAg from the unclarified disruptate, the AE-EBAC (anion-exchange expanded-bed adsorption chromatography) technique was employed. A 54% adsorption and 50.7% recovery of HBcAg were achieved in this direct recovery process. The purity of HBcAg recovered was 49.8%, which corresponds to a purification factor of 2.0. ELISA showed that the HBcAg recovered is functionally active. Portland Press 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17060/1/The%20direct%20recovery%20of%20recombinant%20hepatitis%20B%20core%20antigen%20from%20disruptate%20derived%20from%20continuous.pdf Ho, Chin Woi and Tan, Wen Siang and Kamarudin, Suryani and Ling, Tau Chuan and Tey, Beng Ti (2008) The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling. Biotechnology and Applied Biochemistry, 50 (1). pp. 49-59. ISSN 0885-4513; ESSN: 1470-8744 10.1042/BA20070088 |
| spellingShingle | Ho, Chin Woi Tan, Wen Siang Kamarudin, Suryani Ling, Tau Chuan Tey, Beng Ti The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling |
| title | The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling |
| title_full | The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling |
| title_fullStr | The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling |
| title_full_unstemmed | The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling |
| title_short | The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling |
| title_sort | direct recovery of recombinant hepatitis b core antigen from disruptate derived from continuous-flow bead milling |
| url | http://psasir.upm.edu.my/id/eprint/17060/ http://psasir.upm.edu.my/id/eprint/17060/ http://psasir.upm.edu.my/id/eprint/17060/1/The%20direct%20recovery%20of%20recombinant%20hepatitis%20B%20core%20antigen%20from%20disruptate%20derived%20from%20continuous.pdf |