Surface plasmon resonance biosensor for real-time detection of genetically modified organisms
Application of surface plasmon resonance (SPR) biosensor in detection of genetically modifed organism (GMO) is demonstrated. A total of four biotinylated probes namely Tnosb, P35Sb, LECb and TSQb were successfully immobilized onto the SA chip. Results analysis indicated that the SPR system with the...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/16902/ http://psasir.upm.edu.my/id/eprint/16902/1/IFRJ-2010-477-483_Kqueen%5B1%5D_2.pdf |
| _version_ | 1848843091487752192 |
|---|---|
| author | Cheah, Yoke Kqueen Radu, Son |
| author_facet | Cheah, Yoke Kqueen Radu, Son |
| author_sort | Cheah, Yoke Kqueen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Application of surface plasmon resonance (SPR) biosensor in detection of genetically modifed organism (GMO) is demonstrated. A total of four biotinylated probes namely Tnosb, P35Sb, LECb and TSQb were successfully immobilized onto the SA chip. Results analysis indicated that the SPR system with the sensor chip immobilized with the Tnosb, P35Sb, LECb and TSQb biotinylated probes potentially detect complementary standard fragments as low as 1 nM. Biospecifc interaction analysis (BIA), employing surface plasmon resonance (SPR) and biosensor technologies provide easy, rapid and automatable approach in detection of GMOs. Short
assay times, label free DNA hybridization reaction and no toxic compounds are required, i.e. ethidium bromide, and the reusability of the sensor surface are some of the factors that contribute to the general advantages of the
surface plasmon resonance (SPR) biosensor system in detection of GMOs. |
| first_indexed | 2025-11-15T08:09:31Z |
| format | Article |
| id | upm-16902 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:09:31Z |
| publishDate | 2010 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-169022015-09-29T01:01:06Z http://psasir.upm.edu.my/id/eprint/16902/ Surface plasmon resonance biosensor for real-time detection of genetically modified organisms Cheah, Yoke Kqueen Radu, Son Application of surface plasmon resonance (SPR) biosensor in detection of genetically modifed organism (GMO) is demonstrated. A total of four biotinylated probes namely Tnosb, P35Sb, LECb and TSQb were successfully immobilized onto the SA chip. Results analysis indicated that the SPR system with the sensor chip immobilized with the Tnosb, P35Sb, LECb and TSQb biotinylated probes potentially detect complementary standard fragments as low as 1 nM. Biospecifc interaction analysis (BIA), employing surface plasmon resonance (SPR) and biosensor technologies provide easy, rapid and automatable approach in detection of GMOs. Short assay times, label free DNA hybridization reaction and no toxic compounds are required, i.e. ethidium bromide, and the reusability of the sensor surface are some of the factors that contribute to the general advantages of the surface plasmon resonance (SPR) biosensor system in detection of GMOs. Faculty of Food Science and Technology, Universiti Putra Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16902/1/IFRJ-2010-477-483_Kqueen%5B1%5D_2.pdf Cheah, Yoke Kqueen and Radu, Son (2010) Surface plasmon resonance biosensor for real-time detection of genetically modified organisms. International Food Research Journal, 17 (2). pp. 477-483. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/17%20%2802%29%202010/IFRJ-2010-477-483_Kqueen%5B1%5D.pdf |
| spellingShingle | Cheah, Yoke Kqueen Radu, Son Surface plasmon resonance biosensor for real-time detection of genetically modified organisms |
| title | Surface plasmon resonance biosensor for real-time detection of genetically modified organisms |
| title_full | Surface plasmon resonance biosensor for real-time detection of genetically modified organisms |
| title_fullStr | Surface plasmon resonance biosensor for real-time detection of genetically modified organisms |
| title_full_unstemmed | Surface plasmon resonance biosensor for real-time detection of genetically modified organisms |
| title_short | Surface plasmon resonance biosensor for real-time detection of genetically modified organisms |
| title_sort | surface plasmon resonance biosensor for real-time detection of genetically modified organisms |
| url | http://psasir.upm.edu.my/id/eprint/16902/ http://psasir.upm.edu.my/id/eprint/16902/ http://psasir.upm.edu.my/id/eprint/16902/1/IFRJ-2010-477-483_Kqueen%5B1%5D_2.pdf |