Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere

A study was conducted in in vitro condition to determine the specific growth rate, sugar consumption and generation time of four diazotrophic bacterial species, Burkholderia, Rhizobium and two Corynebacterium sp. in four different carbon substrates. Growth of all bacterial strains was significantly...

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Main Authors: Naher, Umme Aminun, Othman, Radziah, Mohd Saud, Halimi, Shamsuddin, Zulkifli, Ismail, Mohd Razi
Format: Article
Language:English
English
Published: Asian Network for Scientific Information 2008
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/16807/
http://psasir.upm.edu.my/id/eprint/16807/1/Specific%20growth%20rate%20and%20carbon%20sugar%20.pdf
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author Naher, Umme Aminun
Othman, Radziah
Mohd Saud, Halimi
Shamsuddin, Zulkifli
Ismail, Mohd Razi
author_facet Naher, Umme Aminun
Othman, Radziah
Mohd Saud, Halimi
Shamsuddin, Zulkifli
Ismail, Mohd Razi
author_sort Naher, Umme Aminun
building UPM Institutional Repository
collection Online Access
description A study was conducted in in vitro condition to determine the specific growth rate, sugar consumption and generation time of four diazotrophic bacterial species, Burkholderia, Rhizobium and two Corynebacterium sp. in four different carbon substrates. Growth of all bacterial strains was significantly affected by the sugars. Burkholderia sp. (Sb13) rapidly attained maximum population in glucose, fructose and sucrose and the specific growth rate best fitted with exponential model. The cell generation times of Burkholderia sp. (Sb13) in glucose, fructose and sucrose substrate were 0.4, 0.55 and 0.1 h, respectively. Rhizobium sp. (Sb16) followed an exponential growth model in fructose and its generation time was 0.62 h. The growth of Corynebacterium sp. (Sb26) was higher in arabinose and followed logarithmic growth model with generation time of 0.68 h. Corynebacterium sp. (Sb35) showed faster generation time (1 h) in sucrose substrates and the specific growth rate in all four carbon substrates followed logarithmic model of growth phase. Burkholderia sp. and Corynebacterium sp. (Sb26) consumed 100% of arabinose after 36 h of incubation period. The total sugar consumption by the diazotrophs were higher in logarithmic model of growth phase compared to exponential, power and polynomial model.
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institution Universiti Putra Malaysia
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language English
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spelling upm-168072015-12-22T04:49:20Z http://psasir.upm.edu.my/id/eprint/16807/ Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere Naher, Umme Aminun Othman, Radziah Mohd Saud, Halimi Shamsuddin, Zulkifli Ismail, Mohd Razi A study was conducted in in vitro condition to determine the specific growth rate, sugar consumption and generation time of four diazotrophic bacterial species, Burkholderia, Rhizobium and two Corynebacterium sp. in four different carbon substrates. Growth of all bacterial strains was significantly affected by the sugars. Burkholderia sp. (Sb13) rapidly attained maximum population in glucose, fructose and sucrose and the specific growth rate best fitted with exponential model. The cell generation times of Burkholderia sp. (Sb13) in glucose, fructose and sucrose substrate were 0.4, 0.55 and 0.1 h, respectively. Rhizobium sp. (Sb16) followed an exponential growth model in fructose and its generation time was 0.62 h. The growth of Corynebacterium sp. (Sb26) was higher in arabinose and followed logarithmic growth model with generation time of 0.68 h. Corynebacterium sp. (Sb35) showed faster generation time (1 h) in sucrose substrates and the specific growth rate in all four carbon substrates followed logarithmic model of growth phase. Burkholderia sp. and Corynebacterium sp. (Sb26) consumed 100% of arabinose after 36 h of incubation period. The total sugar consumption by the diazotrophs were higher in logarithmic model of growth phase compared to exponential, power and polynomial model. Asian Network for Scientific Information 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16807/1/Specific%20growth%20rate%20and%20carbon%20sugar%20.pdf Naher, Umme Aminun and Othman, Radziah and Mohd Saud, Halimi and Shamsuddin, Zulkifli and Ismail, Mohd Razi (2008) Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere. Journal of Biological Sciences, 8 (6). pp. 1008-1014. ISSN 1727-3048 http://scialert.net/qredirect.php?doi=jbs.2008.1008.1014&linkid=pdf Rice. Rhizosphere. Plants - Microbiology. English
spellingShingle Rice.
Rhizosphere.
Plants - Microbiology.
Naher, Umme Aminun
Othman, Radziah
Mohd Saud, Halimi
Shamsuddin, Zulkifli
Ismail, Mohd Razi
Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere
title Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere
title_full Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere
title_fullStr Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere
title_full_unstemmed Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere
title_short Specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere
title_sort specific growth rate and carbon sugar consumption of diazotrophs isolated from rice rhizosphere
topic Rice.
Rhizosphere.
Plants - Microbiology.
url http://psasir.upm.edu.my/id/eprint/16807/
http://psasir.upm.edu.my/id/eprint/16807/
http://psasir.upm.edu.my/id/eprint/16807/1/Specific%20growth%20rate%20and%20carbon%20sugar%20.pdf