Silylation of mica for lipase immobilization as biocatalysts in esterification
Mica was modified either by acid treatment, grafting with aminopropyl-, octyl-, vinyl-, mercapto- and glycidoxy-triethoxysilanes, and activation of pre-treated support with glutaraldehyde (Glu). The derivatives were characterized by X-ray diffraction (XRD), infra-red spectroscopy (FTIR), surface are...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16712/ http://psasir.upm.edu.my/id/eprint/16712/1/Silylation%20of%20mica%20for%20lipase%20immobilization%20as%20biocatalysts%20in%20esterification.pdf |
| _version_ | 1848843038053367808 |
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| author | Zaidan, Uswatun Hasanah Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Othman, Siti Salhah Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar |
| author_facet | Zaidan, Uswatun Hasanah Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Othman, Siti Salhah Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar |
| author_sort | Zaidan, Uswatun Hasanah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mica was modified either by acid treatment, grafting with aminopropyl-, octyl-, vinyl-, mercapto- and glycidoxy-triethoxysilanes, and activation of pre-treated support with glutaraldehyde (Glu). The derivatives were characterized by X-ray diffraction (XRD), infra-red spectroscopy (FTIR), surface area and porosity analysis, scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX) and transmission electron microscopy (TEM) techniques. The modified micas were used for immobilization of lipase from Candida rugosa (CRL). Activity of the lipase was determined by esterification and exhibited the improved
activity than the free enzyme following the order; Amino-CRLNGlu-Amino-CRLNOctyl-CRLNVinyl-CRLNGlycidoxy CRLNMercapto-CRLNMica-CRL. Lipase immobilized mica showed enhanced protein loading(up to 8.22 mg protein/g support) and immobilization (up to 78%) compared to the free lipase and unmodified mica. |
| first_indexed | 2025-11-15T08:08:40Z |
| format | Article |
| id | upm-16712 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:08:40Z |
| publishDate | 2010 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-167122016-09-02T04:50:06Z http://psasir.upm.edu.my/id/eprint/16712/ Silylation of mica for lipase immobilization as biocatalysts in esterification Zaidan, Uswatun Hasanah Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Othman, Siti Salhah Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Mica was modified either by acid treatment, grafting with aminopropyl-, octyl-, vinyl-, mercapto- and glycidoxy-triethoxysilanes, and activation of pre-treated support with glutaraldehyde (Glu). The derivatives were characterized by X-ray diffraction (XRD), infra-red spectroscopy (FTIR), surface area and porosity analysis, scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX) and transmission electron microscopy (TEM) techniques. The modified micas were used for immobilization of lipase from Candida rugosa (CRL). Activity of the lipase was determined by esterification and exhibited the improved activity than the free enzyme following the order; Amino-CRLNGlu-Amino-CRLNOctyl-CRLNVinyl-CRLNGlycidoxy CRLNMercapto-CRLNMica-CRL. Lipase immobilized mica showed enhanced protein loading(up to 8.22 mg protein/g support) and immobilization (up to 78%) compared to the free lipase and unmodified mica. Elsevier 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16712/1/Silylation%20of%20mica%20for%20lipase%20immobilization%20as%20biocatalysts%20in%20esterification.pdf Zaidan, Uswatun Hasanah and Abdul Rahman, Mohd Basyaruddin and Basri, Mahiran and Othman, Siti Salhah and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2010) Silylation of mica for lipase immobilization as biocatalysts in esterification. Applied Clay Science, 47 (3-4). pp. 276-282. ISSN 0169-1317; ESSN: 1872-9053 10.1016/j.clay.2009.11.004 |
| spellingShingle | Zaidan, Uswatun Hasanah Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Othman, Siti Salhah Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Silylation of mica for lipase immobilization as biocatalysts in esterification |
| title | Silylation of mica for lipase immobilization as biocatalysts in esterification |
| title_full | Silylation of mica for lipase immobilization as biocatalysts in esterification |
| title_fullStr | Silylation of mica for lipase immobilization as biocatalysts in esterification |
| title_full_unstemmed | Silylation of mica for lipase immobilization as biocatalysts in esterification |
| title_short | Silylation of mica for lipase immobilization as biocatalysts in esterification |
| title_sort | silylation of mica for lipase immobilization as biocatalysts in esterification |
| url | http://psasir.upm.edu.my/id/eprint/16712/ http://psasir.upm.edu.my/id/eprint/16712/ http://psasir.upm.edu.my/id/eprint/16712/1/Silylation%20of%20mica%20for%20lipase%20immobilization%20as%20biocatalysts%20in%20esterification.pdf |